A strain of Bacillus amyloliquefaciens sxz-n2 producing huperzine A and huperzine B and its use

Abstract

The invention provides a Bacillus amyloliquefaciens SXZ‑N2 strain producing huperzine A and huperzine B, and the strain can produce huperzine A and huperzine B. The strain, named SXZ‑N2, belongs to Bacillus amyloliquefaciens. The strain is preserved in the China Center for Typical Culture Collection, address: China, Wuhan, Wuhan University; zip code 430072, the preservation date is September 14, 2017, and the preservation number is: CCTCC NO: M2017510; the 16S rDNA sequence of the strain is shown in SEQ ID NO.1 is shown. The above-mentioned Bacillus amyloliquefaciens SXZ‑N2 strain can be used for fermentation to prepare huperzine A and huperzine B. The microbial fermentation conditions are: medium formula: yeast powder 5g / L, peptone 6g / L, sodium chloride 5g / L, sucrose 8g / L, pH 8.0; culture conditions: 32°C, 150r / min, fermentation for 10 days.

Description

technical field [0001] The invention relates to a bacillus amyloliquefaciens strain, which can produce huperzine A and huperzine B, and belongs to the technical field of microorganisms. Background technique [0002] At present, the research on plant endophytes has been paid more and more attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to discover new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that plant endophytes can produce the same or similar active substances and precursors as host plants. This discovery directly promotes the extensive research on plant endophytes, especially medicinal plant endophytes. With people's gradual research on endophytes of medicinal plants, using endophytes that can metabolize and produce corresp...

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Problems solved by technology

However, due to the scarcity of Huperzia serrata plant resources, slow growth, and low content of huperzine A, only relying on the extraction of huperzine A from Huperzia serrata cannot meet the market demand. However, the activity of chemically synthesized huperzine A is far lower. It is better than natural huperzine A, and the cost of obtaining highly optically active huperzine A is relatively high

At present, there are some relevant reports in the field of using Huperzia serrata endophytes for microbial fermentation to produce huperzine A, but the types and yields of these strains still cannot meet the market demand for huperzine A

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