Preparation method and antioxidant application of taro flower water extract

Abstract

The invention discloses a preparation method for the water extract of the flowers of Apios americana Medikus. The preparation method comprises the following steps: 1) mixing the flowers of Apios americana Medikus with water and successively carrying out smashing, ultrasonic extraction and centrifugation to obtain a supernatant; 2) subjecting the supernatant to rotary evaporation and concentrationuntil the volume of the supernatant is reduced by 80 to 90%; 3) subjecting a concentrate obtained in the step 2) to separation and purification via macroporous resin with an aqueous formic acid solution with a volume concentration of 1% as a rinsing agent and an aqueous methanol solution containing 0.1% of formic acid as an eluting agent, and collecting eluate; 4) subjecting the eluate to rotary evaporation and concentration until the volume of the eluate is reduced by 80 to 90% so as to obtain slurry-like concentrate; and 5) pre-freezing the slurry-like concentrate, and then drying the pre-frozen slurry-like concentrate into powder with a vacuum lyophilizer so as to obtain the water extract of the flowers of Apios americana Medikus. The invention also discloses application of the water extract of the flowers of Apios americana Medikus to preparation of inhibitors for oxidative damage to nerve cells.

Description

technical field

[0001] The invention relates to the field of medicine, in particular to a preparation method of a water extract of bean taro flower and its anti-oxidation application. Background technique

[0002] Apios americana Medikus is a perennial leguminous plant (Papilionaceae subfamily) native to the eastern part of North America from Canada to the southern part of Florida. Its edible part is the underground tuber, and its protein content is higher than that of other plant tubers. It is a crop with both nutrition and economic value, so it is often used as a staple food by Indians. Bean taro has been successfully introduced in China since 2009, and has been gradually promoted and planted in Fuyang, Jinhua, Longyou, Wenzhou and other places. Studies have shown that different parts of taro (flowers, vines, leaves, tubers) are rich in free amino acids, soluble proteins, polysaccharides, saponins, flavonoids, isoflavones, vitamin C, vitamin E and minerals. Among them, t...

Images