In-vitro culture method of rubber tree powdery mildew and culture medium for in-vitro culture of rubber tree powdery mildew

Abstract

The invention discloses an in-vitro culture method of rubber tree powdery mildew and a culture medium for in-vitro culture of the rubber tree powdery mildew and belongs to the technical field of plantpathology. The in-vitro culture method of rubber tree powdery mildew comprises steps as follows: A) preparation of a nutrient solution and a culture tray; B) preparation of in-vitro leaves; C) the treated in-vitro leaves are inserted into PCR plate holes filled with the nutrient solution, powdery mildew on the infected rubber tree leaves is slightly brushed with an ink brush or hair brush which is disinfected in 75% alcohol and air-dried to the in-vitro leaves treated by the nutrient solution, the in-vitro leaves are put in an artificial climate box at the humidity of 90% and at the temperature of 23 DEG C and the rubber tree powdery mildew is subjected to light culture for 16 hours and dark culture for 8 hours every day. The in-vitro culture method of the rubber tree powdery mildew is simple, convenient and efficient for a long time, rubber tree powdery mildew can be cultured on a large scale, in-vitro culture of the rubber tree powdery mildew on the leaves is realized, and the problems about culture and storage of the rubber tree powdery mildew are solved.

Description

technical field [0001] The invention belongs to the technical field of plant pathology, and in particular relates to a method for in vitro culture of rubber tree powdery mildew bacteria and a culture medium thereof. Background technique [0002] Rubber tree powdery mildew is one of the most important leaf diseases of Hevea brasiliensis caused by fungus infection of the genus oidium heveae B.A. Steinm. Since it was first discovered in Java, Indonesia in 1918, it has spread all over the world's rubber planting areas. When the disease is serious, the powdery mildew causes the leaves and flowers of the rubber tree to fall, which reduces latex production, weakens the vigor of the rubber tree, and dies new shoots, which has a great impact on the growth and output of the rubber tree. [0003] Rubber tree powdery mildew, as a climate disease, mainly breaks out in cold and humid spring conditions, and rubber tree powdery mildew is an obligate parasite, which cannot grow on artificial...

AI Technical Summary

Problems solved by technology

However, the storage method of bagged seedlings has the disadvantages of large area and less young leaves that can be used, so it is difficult to meet the test requirements.