Tissue vitrification liquid SUT and preparation and application thereof

A transparent and organized technology, applied in transmittance measurement, sampling devices, etc., can solve the problems of different processing time, complex reagent composition, poor transparency effect, etc., to reduce cost and difficulty, increase transparency effect, and improve transparency. Excellent effect

Inactive Publication Date: 2018-01-23
王志伟
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  • Application Information

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Problems solved by technology

However, the birth of various methods is mainly used for the study of the nervous system (brain tissue), and 3DISCO has been used for other organs to be transparent, but it is easy to cause fluorescence quenching
The clearing methods published in the journal CELL in 2014 include CUBIC and PACT-PARS, in which the clearing solution of CUBIC contains two reagents, which need two reagents to treat the tissue in sequence; the clearing solution of PACT-PARS is 8% SDS, two The whole body of the mouse was cleared by perfusing the two clearing fluids through the circulatory system. PACT-PARS also performed confocal scanning imaging of the brain, which vividly displayed the structure of brain neurons. However, research on other organs still remains to be done. superficial
[0008] (1) Although t...

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  • Tissue vitrification liquid SUT and preparation and application thereof
  • Tissue vitrification liquid SUT and preparation and application thereof
  • Tissue vitrification liquid SUT and preparation and application thereof

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with specific examples, so that those skilled in the art can better understand the present invention and implement it, but the given examples are not intended to limit the present invention.

[0035] The preparation method of tissue clearing liquid SUT in the embodiment of the present invention is as follows:

[0036] Weigh 4-12 grams of solid SDS (sodium dodecyl sulfate) and 15-40 grams of Urea (urea) into a glass bottle, add an appropriate amount of 0.1M PBS (distilled water or saline can also be used instead, less than 80ml), Stir and mix, dissolve, add 10-20 ml of liquid TritonX-100, heat and stir evenly, then dilute to 100 ml with 0.1M PBS (distilled water or saline can also be used instead), and stir evenly.

[0037] The composition of the tissue clearing liquid SUT of the embodiment of the present invention is as follows:

[0038] The solute mainly includes SDS, Urea and TritonX-100, and the so...

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Abstract

An embodiment of the invention relates to a tissue vitrification liquid SUT, which mainly comprises SDS, urea and TritonX-100. The invention further relates to a preparation and application of the tissue vitrification liquid SUT. The tissue vitrification liquid SUT provided in the embodiment is reduced in preparation cost and difficulty, is improved in vitrification effect, and has a cardiac muscle vitrification degree superior than that of some vitrification liquids in the prior art.

Description

technical field [0001] The invention relates to a tissue clearing fluid SUT and its preparation and application. Background technique [0002] It has always been a dream of scientists to obtain visualized and complete tissue structure information at the single-cell resolution level. Through single-cell resolution imaging of intact tissues, three-dimensional stereoscopic images of intact tissues can be obtained, so as to clarify the connection mode and dynamic changes between cells at the organ and system level, which will greatly improve our understanding of the occurrence and development of diseases Recognize that single-cell events in disease can ultimately lead to changes in the health of the entire organism. But the opacity of tissues and organs has become the biggest obstacle to realize this dream. [0003] Classical protein qualitative and quantitative techniques (that is, to determine which protein is the content of a certain protein) include western blot, immunohis...

Claims

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Application Information

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IPC IPC(8): G01N21/59G01N1/06
Inventor 王志伟王巍张杰范桄溥闫宇鹏
Owner 王志伟
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