A kind of method of rapid pre-freeze freeze-drying reagent

Abstract

The invention discloses a method for quickly pre-freezing and freeze-drying reagents. First, a pre-freezing plate is prepared, and a plurality of pits with hemispherical bottoms are evenly distributed on the surface of the pre-freezing plate; the inner surface of the pits is coated with a hydrophobic material. layer; when pre-freezing, firstly add the prepared liquid reagent into the pits of the pre-freezing plate in turn, due to the effect of the hydrophobic material layer on the inner surface of the pit, the liquid reagent presents spherical droplets; then immerse the pre-freezing plate in liquid nitrogen, Keep the liquid nitrogen level not submerged on the surface of the pre-freezing plate, and after freezing for 2 to 5 minutes, directly collect the solidified spherical pre-freezing reagent and put it in a freeze dryer for vacuum freeze-drying. The method of the present invention is simple and practical, and the prefreezing speed of the reagent is fast and the time is short; the reagent is not in contact with liquid nitrogen during prefreezing, which ensures the cleanliness of the reagent; the prefreezing reagent is spherical, which greatly increases the sublimation surface area of ​​the freeze-dried reagent and shortens the subsequent Freeze-drying process; pre-frozen reagents can be collected and then freeze-dried in batches, which greatly increases the yield of freeze-dried reagents.

Description

technical field [0001] The invention relates to reagents for molecular biology tests, in particular to a method for rapidly pre-freezing and freeze-drying reagents. Background technique [0002] Freeze-dried reagents refer to reagents prepared by freeze-drying methods, and are generally used for reagents containing active substances that are not resistant to high temperatures. Freeze-dried reagents are generally prepared by freeze-drying technology, that is, substances containing a large amount of water (including proteins, nucleotides, microorganisms, etc.) are pre-cooled and pre-frozen into solids, and then the solid water is directly sublimated under vacuum conditions. out, while the substance itself remains in the ice shelf when it is frozen, so the volume of the reagent remains unchanged after drying; since the entire drying process is carried out at a lower temperature, it is especially suitable for many heat-sensitive substances. After the reagent is freeze-dried, 95...

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Problems solved by technology

Obviously, the pre-freezing time of this kind of freeze-preserved reagent is longer, and the quality of the obtained freeze-dried reagent is not satisfactory

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