Method for inducing plumbago auriculata leaves to directly generate adventitious roots

A technology of indigo and leaves, which is applied in the field of plant cultivation, can solve the problems of long cultivation period, etc., and achieve the effect of early rooting time, fast growth and development, and not limited by the growing season

Inactive Publication Date: 2018-02-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of obtaining blue flower root is still whole plant acquisition (including seedlings and tissue cultured seedlings). The process of bud-rooting, the cultivation period is longer

Method used

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  • Method for inducing plumbago auriculata leaves to directly generate adventitious roots
  • Method for inducing plumbago auriculata leaves to directly generate adventitious roots
  • Method for inducing plumbago auriculata leaves to directly generate adventitious roots

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Embodiment 1

[0018] First cut the leaves from the good plants, brush the surface dust and impurities with a brush, soak the leaves in an ultrasonic cleaning machine with detergent for 0.5h, and then rinse with running water for 2h. Place on the ultra-clean workbench, disinfect with 75% alcohol for 45 seconds, wash with sterile water for 3 to 4 times, then disinfect with 0.1% mercury liters for 5 minutes, wash with bacteria water for 3 to 4 times, and then use 0.1% liters Disinfect mercury for 5 minutes and wash 4 to 5 times with sterile water. Cut the leaves into 1cm×1cm with sterilized scissors, and inoculate them into MS induction medium. The formula is shown in Table 1. The above-mentioned medium contains 30% sucrose, 0.45% agar, pH 5.80-5.85, culture temperature (25±2)°C, light intensity 1500lx, photoperiod (light period h / dark period h) 12 / 12.

[0019] Table 1 Treatment numbers of different hormone ratios

[0020]

[0021] The results after 4 weeks of cultivation are shown in Table 2:

[...

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Abstract

The invention provides a method for inducing plumbago auriculata leaves to directly generate adventitious roots. The method disclosed by the invention avoids single shoot induction and clumpy bud induction treatment and comprises the following steps: cleaning and sterilizing the plumbago auriculata leaves, inoculating the plumbago auriculata leaves into an MS culture medium containing inducing medicament and culturing under the conditions that a temperature is 25+/-2 DEG C, an illumination intensity is 1500lx and a light period/a dark period is 12h/12h. The inducing medicament is NAA or a mixture of NAA and KT, a concentration of the NAA is 0.5 to 1.5mg/L, and a concentration of the KT is 0.1 to 0.3mg/L. The root culturing method disclosed by the invention not only has the advantages of tissue culture, avoids growing season limit at the same time, has a high propagation coefficient and has the more obvious advantages of directly rooting on the leaves, avoiding single bud and clumpy budinducing processes and greatly shortening a culture period.

Description

Technical field [0001] The invention belongs to the technical field of plant cultivation, and specifically relates to a method for inducing the leaves of Lanhuadan to directly generate adventitious roots. Background technique [0002] As a garden plant with high ornamental value, the blue flower plant has its roots (main parts) containing plumbagin, a secondary metabolite naphthoquinone derivative with high medical value. However, at present, there is a serious shortage of both garden applications and biomedical applications of the plant in China. One of the fundamental reasons is the shortage of resources, which cannot meet the raw materials required for development and application. Due to the self-incompatibility of the plant, the natural seed setting rate is extremely low. In addition to the high medical value, people take simple and crude methods to over-exploit this genus at the expense of the entire plant in order to obtain plumbagin. , Resulting in a sharp decline in the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 高素萍雷霆罗良旭胡荻司文慧
Owner SICHUAN AGRI UNIV
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