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Biocontrol bacterium candida intermedia C410 for preventing and controlling gray mold and suspending agent and application thereof

A technology for Candida and gray mold, applied in the application, biocide, fungicide and other directions, can solve the problem of no biological control of gray mold, etc.

Inactive Publication Date: 2018-02-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology can be used for controlling or prevention of diseases caused by yeast (Candidemiaceae). It works through blocking certain parts of DNA that make up these organisms from growing inside cells called cysts.

Problems solved by technology

Technical Problem addressed in this patented technical problem involves reducing or eliminating damage caused by certain insect pests called Botrytis Cinchis during storage and transportation processes due to their ability to infect these crops through wormholts (a genus) and cause symptoms like yellow blinds downstream in plants grown around them. Current methods involve applying synthetic agents containing harmful substances onto stored products, but current approaches may be less effective at protecting against Boletoma Currents. Biology Controls Techniques aim to provide new ways to manage plant health without relying heavily upon traditional treatments involving chemotherapy drugs.

Method used

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  • Biocontrol bacterium candida intermedia C410 for preventing and controlling gray mold and suspending agent and application thereof
  • Biocontrol bacterium candida intermedia C410 for preventing and controlling gray mold and suspending agent and application thereof
  • Biocontrol bacterium candida intermedia C410 for preventing and controlling gray mold and suspending agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Isolation and identification of Candida intertype C410 strain

[0047] 1. Isolation of strains

[0048] The microorganism is a strain of Candida intertype isolated from healthy strawberry leaves in Wuhan, Hubei. The separation method is as follows: take 10g of healthy strawberry leaf samples, cut them into pieces, put them into a sterilized mortar, add 10mL of sterile water containing 0.2% Tween-20, grind them repeatedly to a paste, and then use sterile water Diluted 10 times, 100 times, 1000 times respectively. Filter the diluted solution with two layers of sterilized gauze, and apply the filtrate on a PDA plate containing 0.01% streptomycin, 100 μL / dish, 5 dishes for each concentration, 15 dishes for each sample treatment, at 20°C Cultivate for 2-3 days, pick a single colony and streak it on a fresh PDA plate containing 0.01% streptomycin for purification, and then pick a single colony. The isolated yeast strain is inoculated by streaking on one side of ...

Embodiment 2

[0051] Embodiment 2: the inhibitory action of candida intertype C410 bacterial strain cell suspension to Botrytis cinerea conidia

[0052] In the center of the YPDA plate, use a 5mm diameter puncher to remove the medium to obtain a hole, and pipette 20 μL of different concentrations (3×10 8 , 3×10 7 , 3×10 6 , 6×10 5 and 6×10 4 CFU / mL) of C410 cell suspension was added dropwise to the wells of each dish, and then 20 μL Botrytis cinerea conidia liquid (2×10 5 spores / mL) into the holes of each dish, and the control treatment was to add 20 μL sterile water and 20 μL gray mold conidia liquid (2×10 5 spores / mL). Then put the petri dish under the condition of 20 ℃ for cultivation, observe the colony growth of Botrytis cinerea in the petri dish on the 3rd and 5th day respectively, and record the diameter of the colony by the cross method ( figure 2 ).

Embodiment 3

[0053] Embodiment 3: the inhibitory action of candida intertype C410 strain cell suspension to tomato leaf gray mold

[0054] Tomato material for testing: cooperation 903 (tomato king). Fresh tomato leaves were collected and rinsed with distilled water. After the water droplets on the surface were air-dried naturally, they were placed in a square box covered with moist absorbent paper, and sterilized filter paper pieces with a diameter of 5 mm were dipped in different concentrations (3×10 8 , 3×10 7 , 3×10 6 , 6×10 5 and 6×10 4 CFU / mL) of C410 cell suspension was covered in the center of the leaves, and then 20 μL of Botrytis cinerea conidia liquid (2×10 6 spores / mL) were dropped onto each filter paper sheet. On the filter paper, only 20 μL of Botrytis cinerea conidia solution (2×10 6 spores / mL) were treated as the control. Cover the mouth of the box with plastic wrap, place it under the condition of 20°C for cultivation, observe the incidence of botrytis cinerea on eac...

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Abstract

The invention discloses biocontrol bacterium candida intermedia C410 for preventing and controlling gray mold and a suspending agent and application thereof. The strain is named as candida intermediaC410, and the preservation number of the strain is CCTCC NO:M2017036. The strain candida intermedia C410 inhibits botryis cinerea conidium germination and mycelium growth, and gray mold infecting tomato leaves and fruits is relieved. The cell suspension solution of the strain candida intermedia C410 has the inhibiting effect on gray mold of greenhouse tomatoes. The cell suspension solution of thestrain candida intermedia C410 has the inhibiting effect on gray mold mycelium growth, conidium germination on YPDA flat plates, tomato leaves and fruits and tomato leaf and fruit infection.

Description

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Claims

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Application Information

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Owner HUAZHONG AGRI UNIV
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