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A gene diagnostic kit for diagnosing and predicting bone metastasis of luminal B breast cancer

A gene diagnosis and kit technology, applied in the field of biochemistry, can solve problems such as increasing the burden on patients, high examination costs, and increasing pressure

Inactive Publication Date: 2018-07-20
知几未来(成都)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have different deficiencies, such as high inspection costs, and interventional diagnosis increases the burden on patients
This increases the pressure on routine detection of bone metastases in breast cancer patients

Method used

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  • A gene diagnostic kit for diagnosing and predicting bone metastasis of luminal B breast cancer
  • A gene diagnostic kit for diagnosing and predicting bone metastasis of luminal B breast cancer
  • A gene diagnostic kit for diagnosing and predicting bone metastasis of luminal B breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Bone Metastasis of Luminal A Breast Cancer

[0078] 1. Experimental samples and experimental methods

[0079] 1. Experimental samples

[0080] The test set and validation set of the non-metastasis group of breast cancer in LuminalA type and the bone metastasis group of breast cancer in LuminalA type.

[0081] 2. Total DNA extraction from serum

[0082] Serum DNA was extracted according to the instructions of the DNABlood Midi Kit, and 0.8 mL of serum was used for each sample. The purity of the extracted DNA was detected by an ultraviolet spectrophotometer, and the absorbance A260 / A280 ratio was between 1.7-2.0 for subsequent operations. Calculate the DNA content and store at -70°C for later use.

[0083] 3. DNA sulfite modification and pyrosequencing detection

[0084] DNA sulfite modification:

[0085] Take 1 μg of DNA, perform methylation modification on genomic DNA according to the instructions of DNAMethylation-Goldkit, and store it at -70°C for futur...

Embodiment 2

[0124] Example 2: Bone metastasis of Luminal B breast cancer

[0125] 1. Experimental samples and experimental methods

[0126] 1. Experimental samples

[0127] The test set and validation set of the breast cancer non-metastasis group in Luminal B type and the bone metastasis group of Luminal B breast cancer group.

[0128] 2. Total DNA extraction from serum

[0129] Serum DNA was extracted according to the instructions of the DNABlood Midi Kit, and 0.8 mL of serum was used for each sample. The purity of the extracted DNA was detected by an ultraviolet spectrophotometer, and the absorbance A260 / A280 ratio was between 1.7-2.0 for subsequent operations. Calculate the DNA content and store at -70°C for later use.

[0130] 3. DNA sulfite modification and pyrosequencing detection

[0131] DNA sulfite modification:

[0132] Take 1 μg of DNA, perform methylation modification on genomic DNA according to the instructions of DNA Methylation-Gol dkit, and store it at -70°C for future...

Embodiment 3

[0171] Example 3: Her-2 overexpression breast cancer bone metastasis

[0172] 1. Experimental samples and experimental methods

[0173] 1. Experimental samples

[0174] The test set and validation set of the Her-2 overexpression breast cancer non-metastasis group and the Her-2 overexpression breast cancer bone metastasis group.

[0175] 2. Total DNA extraction from serum

[0176] Serum DNA was extracted according to the instructions of the DNA Blood Midi Kit, and 0.8 mL of serum was used for each sample. The purity of the extracted DNA was detected by an ultraviolet spectrophotometer, and the absorbance A260 / A280 ratio was between 1.7-2.0 for subsequent operations. Calculate the DNA content and store at -70°C for later use.

[0177] 3. DNA sulfite modification and pyrosequencing detection

[0178] DNA sulfite modification:

[0179]Take 1 μg of DNA, perform methylation modification on genomic DNA according to the instructions of DNAMethylation-Goldkit, and store it at -70...

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Abstract

The invention discloses a methylation gene composition and application thereof in preparation of a kit for diagnostic prognostics of Luminal B breast cancer bone metastasis. The invention discovers that serum methylated PITX1 and methylated AMOT can be combined to be used for diagnostic prognostics of Luminal A breast cancer bone metastasis, serum methylated PTPN1 and methylated SLIT2 can be combined to be used for diagnostic prognostics of the Luminal B breast cancer bone metastasis, serum methylated MYLK2, methylated EFEMP1 and methylated SOSTDC1 can be combined to be used for diagnostic prognostics of Her-2 over-expression breast cancer bone metastasis, and serum methylated MYLK3 and methylated SCARA5 can be combined to be used for diagnostic prognostics of triple negative breast cancerbone metastasis. The methylation gene composition is high in accuracy (90% or higher), low in detection cost, non-invasive, convenient and rapid.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to a diagnostic composition and a diagnostic kit, in particular to a methylation gene diagnostic composition and its application in the preparation of a diagnostic kit for diagnosing and predicting bone metastasis of breast cancer of different molecular subtypes. Background technique [0002] Breast cancer is one of the malignant tumors that threaten women's life and health. About 400,000 to 450,000 people die from breast cancer every year (reference: Clinical characteristics and prognosis analysis of patients with bone metastasis of breast cancer with different molecular subtypes, Journal of Xi'an Jiaotong University· Medical Edition, Volume 38, Issue 5, September 2017). Breast cancer is very prone to distant metastasis, and bone is the most common distant metastasis site of breast cancer, and more than 50% of patients' first metastatic site is bone tissue (reference: Genes associated wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 薛守海李宜健童强刘敏涛胡雨祝
Owner 知几未来(成都)生物科技有限公司