Biogas fermentation promotion bacterium agent

A biogas fermentation and accelerator technology, applied in the fields of fermentation, bacteria, microorganisms, etc., can solve the problems of difficulty in obtaining strains, strict separation and cultivation work, affecting popularization and application, etc., and achieve the effect of easy acquisition.

Active Publication Date: 2018-02-23
BIOGAS SCI RES INST MIN OF AGRI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the research in this field is not yet mature in terms of bacterial agent accelerators, and there is still a lot of room for improvement in the technical effects of gas production efficiency, gas production cycle and raw material adaptability
In addition, although some accelerators have good effects, the strains used are difficult to obtain or require rigorous isolation and cultivation, which affects their popularization and application.

Method used

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  • Biogas fermentation promotion bacterium agent
  • Biogas fermentation promotion bacterium agent
  • Biogas fermentation promotion bacterium agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Fresh pig manure is used as a fermentation substrate, and the fermentation concentration is 2% and added to clean water. The various functional microorganisms in the promoting bacteria agent are counted by volume parts, respectively: the volume parts of the culture solution of the rumen proteolytic bacteria are 8 parts, the culture solution volume parts of the syntrophomonas palmitic acid are 4 parts, and the Dashima The volume fraction of the culture solution of Thermobacter faecalis is 8 parts, the volume fraction of the culture solution of Cellulophilus macroji is 8 parts, the volume fraction of the culture solution of Proteobacter glycolyticum is 12 parts, and the culture solution of Spirochete spirochete The parts by volume are 5 parts, the parts by volume of the culture solution of Tunisia effluent togae are 5 parts by volume, the parts by volume of the culture solution of Enterobacter aerogenes are 5 parts, the parts by volume of the culture solution of Aminobacte...

Embodiment 2

[0050] Rice straw is used as a fermentation substrate, and the fermentation concentration is 1% and added to clean water. The volume ratios of various functional microorganisms in the promoting bacteria agent are as follows: 5 parts by volume of the culture solution of rumen proteolytic bacteria, 5 parts by volume of culture solution of Syntrophomonas palmiticum, and 5 parts by volume of culture solution of Oshima fecal heat The volume fraction of the culture solution of Bacillus is 5 parts, the volume fraction of the culture solution of Cellulophilicum macroji is 12 parts, the volume fraction of the culture solution of Bacillus Glycolyticum Proteolyticum is 8 parts, and the volume fraction of the culture solution of Spirochete spirochetes The number is 5 parts, the volume part of the culture solution of Tunisia effluent gown is 6 parts, the volume part of the culture solution of Enterobacter aerogenes is 5 parts, the volume part of the culture solution of Aminobacterium Columb...

Embodiment 3

[0056] Fresh cow dung is used as a fermentation substrate, and the fermentation concentration is 2% and added to clean water. The volume ratio of each functional microorganism in the promoting bacteria agent is respectively: the volume fraction of the culture solution of rumen proteolytic bacteria is 8 parts, the culture solution volume fraction of Syntrophomonas palmiticum is 4 parts, and the volume ratio of Oshima fecal heat The volume fraction of the culture solution of Bacillus is 7 parts, the volume fraction of the culture solution of Cellulophilus gigantea is 9 parts, the volume fraction of the culture solution of Glycolytica Proteobacterium is 10 parts, and the volume fraction of the culture solution of Spirochete spirochetes The number is 5 parts, the volume parts of the culture solution of Tunisia effluent toga is 5 parts, the volume parts of the culture solution of Enterobacter aerogenes is 5 parts, the volume parts of the culture solution of Aminobacterium Columbia a...

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Abstract

The invention provides a biogas fermentation promotion agent. The promotion agent is prepared from a following microorganism culture solution according to the volume part of the microorganism culturesolution, 15 to 20 parts of firmicutes, 15 to 20 parts of bacteroides, 3 to 5 parts of spirochaeta, 4 to 6 parts of thermotoga, 3 to 5 parts of deltaproteo bacteria, 3 to 5 parts of synergistetes, 3 to 5 parts of cellulomonas and 35 to 40 parts of euryarchaeota. According to the biogas fermentation promotion agent provided by the invention, selected 13 anaerobic fermentation functional strains arepreserved in strain preservation centers at home and abroad and are easy to obtain, culture and compound; according to the fermentation promotion agent provided by the invention, various microorganisms are matched reasonably and can be adaptive to fermentation raw materials with different types so that various raw materials can be rapidly degraded and converted into biogas; according to the biogas fermentation promotion agent, various microorganisms have the same or similar growth culture conditions and the promotion bacterium agent can normally grow and metabolize under the same environmentcondition and play the largest role.

Description

technical field [0001] The invention relates to resource utilization of organic waste, in particular to a biogas fermentation-promoting bacterial agent, which belongs to the fields of biological fermentation technology and renewable energy. Background technique [0002] As a renewable resource of ground biomass, biogas has a long history of use. However, with the increasing demand for economical and scientific biogas production technology, many related technologies have been developed. Among them, the research on biogas accelerator is a hotspot. [0003] At present, the main accelerators can be simply divided into chemical substance accelerators and bacterial agent accelerators. [0004] The main research and development idea of ​​chemical substance accelerators is to promote the growth of methanogens and improve their gas production efficiency, such as the Chinese patent with the authorization number CN 103642846B and the Chinese patent with the publication number CN10292...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P39/00C12P5/02C12R1/01
CPCC12N1/20C12P5/023C12P39/00Y02E50/30
Inventor 李强马诗淳尹小波邓宇周正马泓若曹杰
Owner BIOGAS SCI RES INST MIN OF AGRI
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