A kind of construction method and application of dna large fragment library
A construction method and large fragment technology, applied in the field of molecular biology, can solve the problems of low effective data rate and short read length, and achieve the effect of improving the effective data rate
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[0073] Example 1 DNA Large Fragment Library Construction
[0074] 1. Large fragment processing of DNA samples
[0075] 1.1 Take 2 mL of whole blood from healthy people and extract it according to the operating instructions of the blood genome DNA extraction system (0.1-20 mL) (Tiangen Biochemical Technology Co., Ltd.) to obtain DNA samples. Take 10 μg of DNA sample, use the HydroShear Plus DNA Fragmentation Instrument to fragment large fragments of DNA samples according to the instructions, and the target large fragment length is 10kb.
[0076] 1.2 Prepare 0.8% agarose gel, use TIANGEN company 1Kb DNA Ladder as the molecular weight standard, and electrophoresis at 100V for 2 hours. After electrophoresis, the gel was taken out and stained in TAE containing EB dye for 20 minutes. Fragments around 9-12kb were cut and glued under ultraviolet irradiation.
[0077] 1.3 Put the excised gel into a clean 2.0mL centrifuge tube that has been weighed, and use the agarose gel extractio...
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