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Primer ACY of Ty-3 molecular marker and indexing method thereof

A molecular marker and marker primer technology, applied in the field of bioengineering, can solve the problems of false positives, time-consuming genotyping, and high cost, and achieve the effects of simple operation, guaranteed labeling results, and time and cost savings.

Pending Publication Date: 2018-03-13
江苏绿港现代农业发展股份有限公司
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AI Technical Summary

Problems solved by technology

However, there is a certain distance between most molecular markers and resistance genes, and false positives may occur due to the exchange of linked markers and resistance genes during the marking process (Ji et al., 2007, 2012)
In addition, some markers, such as CAPS markers, require two lengthy steps of PCR amplification and restriction endonuclease digestion of the amplified products in practical applications, which makes their genotyping time-consuming and expensive

Method used

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  • Primer ACY of Ty-3 molecular marker and indexing method thereof
  • Primer ACY of Ty-3 molecular marker and indexing method thereof
  • Primer ACY of Ty-3 molecular marker and indexing method thereof

Examples

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Embodiment 1

[0023] In this example, according to the genome sequences of wild tomato (Solanum pennellii) and cultivated tomato (Solanum lycopersicum), the sequences of the Ty-3 candidate gene Solyc06g051170 were compared in the NCBI database using the BLAST tool, and it was found that there were single bases and 2 bases , 3 bases, 4 bases, or even 9 bases, 10 bases. According to the deletion of 9 bases and 10 bases, use the designed primers to carry out PCR amplification with 6 disease-resistant commercial varieties and 6 susceptible commercial varieties. It is found that the primers designed according to the 10bp deletion can well distinguish the resistant Susceptible varieties, and the band pattern is obvious and clear, the band size of the amplified resistant variety is 132bp, and the fragment size of the susceptible variety is 123bp. It is predicted that the InDel marker is located on tomato chromosome 6, the position of the fourth intron of the Ty-3 candidate gene solyc06g051170 frag...

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Abstract

The invention relates to a primer ACY of a Ty-3 molecular marker and an indexing method thereof. Genome DNA of tomato leaves of two anti-infection materials of a tomato yellow leaf curl virus is subjected to PCR amplification, so that an accurate and efficient molecular marker for distinguishing a disease-resistant variety from a susceptible variety is obtained, and the marker is stable and reliable, and therefore, tomato marker-assistant selective breeding is facilitated. The special Ty-3 molecular marker amplifies a 132bp stripe in the disease-resistant variety and amplifies a 123bp stripe in the susceptible variety, and the stripes are clear; and a primer is designed in a gene region, so that false positive generated by exchange is not generated, and accurate reliability of identification results is guaranteed; and the identification results are completely consistent with a field phenotype of a used material.

Description

technical field [0001] The invention relates to a Ty-3 molecularly marked primer ACY and an indexing method thereof, belonging to the technical field of bioengineering. Background technique [0002] Tomato yellow leaf curl disease (TYLCD) is caused by tomato yellow leaf curl virus (TYLCV) in the Geminiviridae family devastating disease. TYLCD was first reported in Israel in the late 1930s, and in the 1960s, the disease broke out on tomatoes in the Mediterranean Basin (Antignus et al. 1994), and subsequently spread to Africa, Europe, Asia, the Caribbean, and North America. , the occurrence of this disease often causes huge yield losses in tomato, up to 100% (Antignus et al. 1994; Pico et al. 1996; Czosnek et al. 1997; Polston et al. 1997; Delatte et al. 2005). Chinese tomato yellow leaf curl disease was first discovered in Nanning, Guangxi in 1998 (Liu Yule et al., 1998), and then TYLCD also occurred in different tomato growing areas in China. For a long time, TYLCD has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 阿门卢霞李永博张文婷司龙亭
Owner 江苏绿港现代农业发展股份有限公司
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