Method for extracting oligosaccharide from water-chestnut

A technology of oligosaccharides and horseshoes, applied in the fields of oligosaccharides, chemical instruments and methods, sugar derivatives, etc., can solve the problems of oligosaccharides that have not been reported, and achieve the improvement of deep processing capacity of horseshoes, high extraction efficiency, and easy control. Effect

Inactive Publication Date: 2018-03-16
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign studies on horseshoe mainly focus on the extraction and functional identification of flavonoids, polyphenols, sterols, pigments, etc. Research on the extraction of oligosaccharides has not been reported so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method of extracting oligosaccharides from horseshoe of the present invention, comprises the following steps:

[0021] (1) Raw material processing: Wash the fresh water chestnuts, dry the slices at about 55°C, pulverize them in a pulverizer, and filter them through an 80-mesh standard sieve to obtain horseshoe powder.

[0022] (2) ethanol dissolution: the water chestnut powder of step (1) gained is dissolved with ethanol solution, and the mass concentration of ethanol solution is 25%, and solid-liquid ratio (the ratio of horseshoe powder and ethanol solution) is 1g: 35mL, obtains mixed solution.

[0023] (3) Ultrasonic extraction: the mixed solution obtained in step (2) is extracted by ultrasonic, the ultrasonic power is 350W, the extraction time is 24min, centrifuged to obtain the extraction solution.

[0024] (4) Concentration under reduced pressure: Concentrate the extract solution obtained in step (3) under reduced pressure at 50° C. to remove the ethanol solvent ...

Embodiment 2

[0029] A method of extracting oligosaccharides from horseshoe of the present invention, comprises the following steps:

[0030] (1) Raw material processing: Wash the fresh water chestnuts, dry the slices at about 55°C, pulverize them in a pulverizer, and filter them through an 80-mesh standard sieve to obtain horseshoe powder.

[0031] (2) ethanol dissolution: the water chestnut powder of step (1) gained is dissolved with ethanol solution, and the mass concentration of ethanol solution is 30%, and solid-liquid ratio (the ratio of horseshoe powder and ethanol solution) is 1g: 40mL, obtains mixed solution.

[0032] (3) Ultrasonic extraction: the mixed solution obtained in step (2) is extracted by ultrasonic, the ultrasonic power is 400W, the extraction time is 32min, centrifuged to obtain the extraction solution.

[0033] (4) Concentration under reduced pressure: Concentrate the extract solution obtained in step (3) under reduced pressure at 50° C. to remove the ethanol solvent ...

Embodiment 3

[0038] A method of extracting oligosaccharides from horseshoe of the present invention, comprises the following steps:

[0039] (1) Raw material processing: Wash the fresh water chestnuts, dry the slices at about 55°C, pulverize them in a pulverizer, and filter them through an 80-mesh standard sieve to obtain horseshoe powder.

[0040] (2) ethanol dissolution: the horseshoe powder of step (1) gained is dissolved with ethanol solution, and the mass concentration of ethanol solution is 35%, and solid-liquid ratio (the ratio of horseshoe powder and ethanol solution) is 1g: 45mL, obtains mixed solution.

[0041] (3) Ultrasonic extraction: adopt the mixed solution obtained in step (2) of ultrasonic extraction, the ultrasonic extraction power is 450W, the extraction time is 40min, centrifuge to obtain the extraction solution.

[0042] (4) Concentration under reduced pressure: Concentrate the extract solution obtained in step (3) under reduced pressure at 50° C. to remove the ethanol...

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PUM

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Abstract

The invention discloses a method for extracting oligosaccharide from water-chestnut. The method comprises the following steps: (1) dissolving water-chestnut powder in an ethanol solution, and carryingout ultrasonic extraction and centrifuging so as to obtain an extract liquid; (2) subjecting the extract liquid obtained in the step (1) to vacuum concentration and removing ethanol from the extractliquid so as to obtain a concentrated liquid; (3) filtering the concentrated liquid obtained in the step (2) by adopting an ultrafiltration membrane, fitering the obtained permeated liquid through a nanofiltration membrane so as to obtain a retentate; and (4) subjecting the retentate obtained in the step (3) to vacuum concentration until a brix degree is 55 to 65, and carrying out freeze-drying soas to obtain water-chestnut oligosaccharide. The method provided by the invention has the advantages of no pollution, no solvent residue, no generation of phase transition, high extraction efficiency, simple operation, convenience in control, etc.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and in particular relates to a method for extracting oligosaccharides from horseshoes. Background technique [0002] Horseshoe (Eleochairistoberosa) is native to southern my country, also known as water chestnut, ground chestnut, black tea, scorpion grass, etc., belonging to Cyperaceae perennial herb. my country's horseshoe production accounts for 95% of the world's total, and Guangxi's horseshoe production accounts for 70% of the country's horseshoe production. Hezhou, Guangxi is known as the "Hometown of Chinese Horseshoes". Horseshoe is rich in nutrition, juicy and sweet, and has been known as "Underground Sydney" since ancient times. Horseshoe not only has medicinal value, but also has multiple health functions. It is a kind of fruit and vegetable food with both medicine and food. In recent years, the research and development of biological functional compone...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H3/06C08B37/00
CPCC07H1/08C07H3/06C08B37/0003
Inventor 帅良廖玲燕段振华朱东建杨金凤宋慕波康超
Owner HEZHOU UNIV
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