Cultivation method for new variety of Chinese herbaceous peony

A breeding method and technology of new varieties, applied in the field of cultivating new varieties of peony, can solve the problems of affecting the mutation rate and mutagenesis survival rate, low probability of new varieties of peony, and difficulty in producing flower and color varieties, so as to improve seed cell activity and germination efficiency, increase seed cell activity, improve nutrient absorption and resistance

Inactive Publication Date: 2018-03-20
蚌埠宏瑞园林有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims at the existing problems: the traditional breeding method of peony includes sowing, cutting and layering, all of which have good genetic stability, but the natural variation rate is low, the probability of producing new varieties of peony is low, and the breeding of new varieties is not used; However, the hybridization method is limited to hybridization among existing varieties, and the types of flower colors produced are limited, and the range of flower color changes is small, so it is difficult to produce varieties with distinctive characteristics; while in the mutagenesis method, the activity of the seeds and the self-protection ability of the seeds are both low. Will affect the mutation rate and mutagenesis survival rate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0016] A method for cultivating new varieties of peony, comprising the following steps:

[0017] (1) Storing at constant temperature: first sterilize and dip the peony seeds, and then put them in an incubator for storage at a temperature of 45°C and a humidity of 81% for 4.5 hours. Prepare preserved seeds;

[0018] (2) Ionization activation: Immerse the preserved seeds in the inducer for ionization. The colchicine, aloperine and galantamine contained in the inducer can increase the mutation probability of the seeds. Treat them under the conditions of voltage 47V and current 0.64A 38min, the alternating frequency of the positive and negative poles is 6 times / min, the seeds are stimulated by the alternating electric current, the activity of the seed cells is improved, and the mutagenesis rate of the highly active seeds will be higher, and the ionized seeds will be obtained;

[0019] (3) Variable temperature mutagenesis: immerse the ionized seeds in the protective solution and p...

Embodiment 2

[0026] (1) Storing at constant temperature: first sterilize and dip the peony seeds, and then put them in an incubator for storage at a temperature of 47°C and a humidity of 84%, and store them for 5.5 hours, which can make the seeds quickly absorb water and expand, and improve the activity and germination rate of seed cells. Prepare preserved seeds;

[0027] (2) Ionization activation: Immerse the preserved seeds in the inducer for ionization. The colchicine, aloperine and galantamine contained in the inducer can increase the mutation probability of the seeds, and treat them under the conditions of voltage 49V and current 0.72A 39 minutes, the alternating frequency of positive and negative poles is 6 times / min, and the seeds are stimulated by constantly changing the electric current to improve the activity of seed cells, and the mutagenesis rate of highly active seeds will be higher, and ionized seeds will be obtained;

[0028] (3) Variable temperature mutagenesis: immerse the...

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Abstract

The invention discloses a cultivation method for a new variety of Chinese herbaceous peony. The method is characterized by comprising the following steps of preservation at constant temperature, wherein firstly, Chinese herbaceous peony seeds are sterilized and soaked, and then put into a constant-temperature tank and preserved; ionization activation, wherein the preserved seeds are soaked into aninductive agent and ionized; mutagenesis at variable temperature, wherein the ionized seeds are soaked into protective liquid, and the mixture is put into a variable-temperature tank and subjected tovariable temperature processing; cultivation in a greenhouse, wherein the mutagenesis seeds are sowed to a seedling bed, covered with soil, watered with the protective liquid, and cultivated in the greenhouse, and ultraviolet light is used for irradiating the seeds at the seed germination stage; hybridization, wherein Chinese herbaceous peony seedlings are transplanted in early March of the nextyear, Chinese herbaceous peony variant plants are obtained, original Chinese herbaceous peony plants are used as female parents, the Chinese herbaceous peony variant plants are used as male parents, first-generation Chinese herbaceous peony is obtained after the hybridization, the first-generation Chinese herbaceous peony and the variant plants are hybridized to obtain second-generation Chinese herbaceous peony, the second-generation Chinese herbaceous peony is subjected to intraspecific cultivation, and new variety seeds are obtained and cultivated.

Description

technical field [0001] The invention belongs to the technical field of breeding new varieties of flowers, and in particular relates to a method for cultivating new varieties of peony. Background technique [0002] Peony is a herbaceous plant belonging to Paeoniaceae Paeoniaceae for many years. Oblong or pointed round; peony flower color varieties include white, pink, red, purple, yellow, green, black and multiple colors, etc., divided into single flower and Taiwan pavilion. In my country, its wild plants are mostly distributed in Jiangsu, In Northeast China, North China, Shaanxi and southern Gansu, it is mostly found in high-altitude hillside grasslands. Its flowers are gorgeous and similar to peonies. They are mainly planted in botanical gardens, flower beds, gardens, and courtyards to enhance the landscape. ornamental value. Peony can be used as medicine. The paeoniflorin and benzoic acid contained therein have antispasmodic, analgesic, and menstrual effects. Its seeds can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/60A01H1/02A01H1/06A01C1/00A01C1/08
CPCA01H1/02A01C1/00A01C1/08A01H1/06
Inventor 巨刚
Owner 蚌埠宏瑞园林有限公司
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