Preparation method of artificial blood vessel covered by gene editing mesenchymal stem cell
A technology of stem cells and artificial blood vessels, applied in the medical field, can solve problems affecting the treatment effect of artificial blood vessels, achieve the effect of increasing the effect, promoting angiogenesis, and improving the inflammatory response
Inactive Publication Date: 2018-03-23
SUN YAT SEN UNIV
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Problems solved by technology
In summary, the existing problems in the prior art are: the local inflammation after the existing artificial blood vessel transplantation affects the therapeutic effect of the artificial blood vessel
At present, there is no relevant literature to report the use of GREM1 overexpression MSCs cells to improve artificial blood vessels
Method used
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preparation example Construction
[0029] like figure 1 As shown, the method for preparing artificial blood vessels covering gene-edited mesenchymal stem cells provided by the embodiments of the present invention includes:
[0030] S101: constructing a vector for overexpressing GREM1 gene.
[0031] S102: Obtaining GREM1 overexpressed mesenchymal stem cells.
[0032] S103: putting the GREM1-overexpressed mesenchymal stem cell suspension and the artificial blood vessel together into a pulsating bioreactor for culture.
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Abstract
The invention belongs to the technical field of medicine, and discloses a preparation method of an artificial blood vessel covered by a gene editing mesenchymal stem cell. The method comprises the steps of building a carrier of an overexpression GREM1 gene; acquiring the mesenchymal stem cell overexpressed by GREM1; jointly placing a GREM1 overexpressed mesenchymal stem cell suspension and the artificial blood vessel into a pulsatile bioreactor for culturing. According to the artificial blood vessel covered by the gene editing mesenchymal stem cell, provided by the invention, the overexpression GREM1 gene can promote mesenchymal stem cell survival, the covered mesenchymal stem cell and secretory factors thereof can inhibit posttransplant inflammation, angiogenesis is promoted, lower limb ischemia is relieved and repaired, and a new way is provided for treating ischemic diseases.
Description
technical field [0001] The invention relates to a method for preparing an artificial blood vessel covered with gene-edited mesenchymal stem cells, and belongs to the technical field of medicine. Background technique [0002] Peripheral arterial disease (PAD) has an estimated global population burden of 202 million people. Those with critical limb ischemia (CLI), advanced stages of ischemic rest pain, ulceration, and gangrene, are at increased risk for cardiovascular events and adverse limb events, including surgery or amputation. Artificial blood vessels are substitutes for many severely stenotic or occlusive blood vessels, mostly made of synthetic materials such as nylon, polyester (Dacron), and polytetrafluoroethylene (PTFE). The basal layer of the artificial blood vessel is composed of a porous fiber wall. The porous structure can keep the artificial blood vessel sufficiently tight to the blood to avoid excessive blood loss during the operation, and at the same time allo...
Claims
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IPC IPC(8): C12N5/10C12N15/85C12N5/0775C12N5/071
CPCC07K14/51C12N5/0662C12N5/0691
Inventor 向秋玲
Owner SUN YAT SEN UNIV