Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Prostate cancer-specific exosome, IncRNA and preparation methods and application thereof

A technology for prostate cancer and exosomes, applied in the direction of DNA preparation, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve the effect of improving sensitivity and specificity

Active Publication Date: 2018-03-23
WUHAN UNIV
View PDF5 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the in-depth study of the nucleic acid components inside exosomes, the lncRNA in exosomes has opened the door as a new marker for tumor diagnosis and treatment, but in the prior art, there is still a lack of lncRNA pairs in prostate cancer tumor-specific exosomes Application of technology in the diagnosis of prostate cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prostate cancer-specific exosome, IncRNA and preparation methods and application thereof
  • Prostate cancer-specific exosome, IncRNA and preparation methods and application thereof
  • Prostate cancer-specific exosome, IncRNA and preparation methods and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A total of 110 plasma samples were collected, including plasma from 34 patients with histopathologically confirmed prostate cancer, 46 patients with benign prostatic hyperplasia and 30 healthy controls. All plasma samples were obtained from Zhongnan Hospital of Wuhan University, and hemolytic, lipemic, and other abnormal sera were excluded.

[0048] The specific detection steps are as follows:

[0049] 1. Blood sample pretreatment and storage

[0050] Collect the venous blood of the above subjects in EDTA anticoagulant tubes, after standing still, draw the upper plasma into the EP tube, then centrifuge at 2000g at room temperature for 20 minutes, collect the supernatant in a new EP tube, centrifuge at 10000g at room temperature for 20min, draw The supernatant was stored in an enzyme-free EP tube at -80°C for future use.

[0051] 2. Extraction and storage of total exosomes in plasma (Total Exosome Isolation Kit, Cat. No. 4484450, Invitrogen)

[0052] If the plasma sam...

Embodiment 2

[0102] Example 2 Preparation of long-chain non-coding RNASAP30L-AS1 and SChLAP1 kits for the diagnosis of prostate cancer patients

[0103] 1. Real-time fluorescence quantitative PCR specific primer sequence

[0104] SAP30L-AS1 forward primer: 5'-TGAATGGGCTCACCTGTTCC-3'

[0105] SAP30L-AS1 reverse primer: 5'-AGGTCCGGAAGGGAGACTTT-3'

[0106] SChLAP1 forward primer: 5'-TGGACACAATTTCAAGTCCTCA-3'

[0107] SChLAP1 reverse primer: 5'-CATGGTGAAAGTGCCTTATACA-3'

[0108] 2. The double antibody-coupled immunomagnetic beads prepared in Example 1

[0109] 3. Total exosome extraction reagent

[0110] 4. Reagents for reverse transcription

[0111] 5. RT-qPCR reagents

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a prostate cancer-specific exosome. The invention further discloses the prostate cancer-specific exosome and application thereof. The invention furtherdiscloses a preparation method of a long-chain non-coding RNA SAP30L-AS1 and SChLAP1 in the prostate cancer exosome. The invention further discloses application of a reagent for assaying expression quantities of the long-chain non-coding RNA SAP30L-AS1 and SChLAP1 in the prostate cancer exosome in the preparation of a prostate cancer diagnosis reagent. The invention further discloses a prostate cancer diagnosis kit. The prostate cancer-specific exosome in the plasma of a patient with prostate cancer can be successfully separated, and the long-chain non-coding RNA SAP30L-AS1 and SChLAP1 can bedetected. The diagnostic efficacy and sensitivity and specificity are all remarkably increased.

Description

technical field [0001] The invention belongs to the field of biotechnology and tumor molecular biology technology, and specifically relates to prostate cancer-specific exosomes, lncRNA and their preparation methods and applications, and in particular to the separation of tumor sources in the plasma of prostate cancer patients by using double-antibody coupling immunomagnetic beads Exosomes and their lncRNA SAP30L-AS1 and lncRNA SChLAP1 are used as diagnostic markers of prostate cancer and kits for early diagnosis of prostate cancer. Background technique [0002] According to the latest cancer statistics analysis, prostate cancer (PCa) is one of the leading causes of male cancer death in the world. In 2016, new prostate cancer cases in the United States accounted for about 21% of the number of newly diagnosed cancer patients. The rate has also risen rapidly. The main treatment for prostate cancer at early stages is castration therapy, but progresses to castration-resistant pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/10C12N15/11C12Q1/6886G01N33/574G01N33/543
CPCC12N15/10C12N15/11C12N2310/00C12Q1/6886C12Q2600/178G01N33/54326G01N33/57434
Inventor 汪付兵王宇慧陈浩
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com