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Method for extracting isoquercitrin from longhairy antenoron herb

A technology of isoquercitrin and desmodium, which is applied in the field of extraction in the field of biotechnology, can solve the problems of low selectivity, oil loss of aromatic compounds, and unsatisfactory extraction effect

Inactive Publication Date: 2018-03-27
桂林纽泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting isoquercitrin from longhairy antenoron herb
  • Method for extracting isoquercitrin from longhairy antenoron herb
  • Method for extracting isoquercitrin from longhairy antenoron herb

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The method for extracting isoquercitrin from Desmodium, specifically comprises the following steps:

[0025] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 20-mesh sieve;

[0026] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0027] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, and ...

Embodiment 2

[0029] The method for extracting isoquercitrin from Desmodium, specifically comprises the following steps:

[0030] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 40-mesh sieve;

[0031] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0032] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous resin,...

Embodiment 3

[0034] The method for extracting isoquercitrin from Desmodium, specifically comprises the following steps:

[0035]1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through an 80-mesh sieve;

[0036] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 185-200°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0037] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, and l...

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Abstract

The invention discloses a method for extracting isoquercitrin from Desmodium, comprising the following steps: 1) Cleaning Desmodium, drying, pulverizing with a pulverizer, and sieving; 2) Subcritical water extraction : Put the processed raw materials in the extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle, extract the temperature at 185‑200 ° C, the pressure is 8‑10 Mpa, and the extraction time is 0.5 ‑1.0h, extract Desmodium, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained; 3) Purification: the extract obtained in step 2) is concentrated, and the column is carried out through a macroporous resin Chromatography, eluting with 50% v / v ethanol, the eluate was obtained, concentrated and dried to obtain the finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting isoquercitrin from Desmodium. Background technique [0002] Isoquercitrin, alias: quercetin-3-O-glucoside, isoquercetin, apocynum. Molecular formula: C 21 h 20 o 12 , molecular weight: 464.38. Yellow needle-like crystals (water), melting point 225-227°C, almost insoluble in cold water, slightly soluble in boiling water, soluble in alkali solution to appear dark yellow. Yellow crystal (ethanol), melting point 206-208°C and 232-236°C. Pale yellow needle crystal, melting point 220-222°C (decomposition). Crystallization (hot water), melting point 230°C. Animal experiments have hypotensive effect. Tests such as capillary permeability have shown anti-inflammatory effects. It has a poisonous effect on the larvae of the cotton budworm. It has enzyme-lowering effect and is one of the active ingredients of Tian Jihuang in treating hepat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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