Simple, convenient and rapid determination method of non-sugar solids in yellow rice wine with alcohol concentration of 12-14%

A non-sugar solids, rapid determination technology, used in the preparation of test samples, color/spectral property measurement, etc. Good accuracy and reliability, simple and rapid operation, and low cost of detection

Inactive Publication Date: 2018-03-27
陈荷冰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current national standard of rice wine uses the 4-hour drying method to measure the non-sugar solid content in the wine sample. This method takes a long time, and there are cases where the quality of the wine sample does not reach a constant weight after drying for 4 hours, which is not conducive to rapid determination. , precise measurement and batch testing
In addition, there is a rapid determination method for non-sugar solids in wine samples based on near-infrared spectroscopy, but this method requires expensive equipment and high detection costs

Method used

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  • Simple, convenient and rapid determination method of non-sugar solids in yellow rice wine with alcohol concentration of 12-14%

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 is the preparation of a standard curve for the determination of the mass-to-volume ratio of water and ethanol in the simple and rapid determination method for non-sugar solids in rice wine with 12 to 14 degrees of the present invention.

[0019] (1) Preparation of artificial ethanol water system

[0020] Use a pipette to measure 12.00ml, 12.40ml, 12.80ml, 13.20ml, 13.60ml and 14.00ml of absolute ethanol into six 100ml volumetric flasks, and place the volumetric flasks on the analytical balance to add 88.00g and 87.60g respectively , 87.20g, 86.80g, 86.40g, and 86.00g of distilled water, tightly capped the volumetric bottle cap, repeatedly inverted and fully mixed, left at room temperature for 10 minutes, and finally obtained the mass-volume ratio of water and ethanol, that is, the mass of water / volume of ethanol (corresponding to 7.333, 7.065, 6.812, 6.576, 6.353 and 6.143) artificial ethanol water system.

[0021] (2) Pretreatment of artificial ethanol water...

Embodiment 2

[0030] Example 2 is the actual determination of the non-sugar solids of rice wine samples marked with an alcohol content of 12 by using the new method of the present invention.

[0031] (1) Select commercially available Yuejing eight-year-old Shanghai old wine (produced by Shaoxing County Yuejing Liquor Co., Ltd.), with an alcohol content of 12% marked on the bottle. For the sake of accuracy, the alcohol content needs to be measured again, so based on the current national standard of rice wine, the alcohol content of this rice wine is determined to be 12.2 by the alcohol meter method. After weighing and conversion, the mass of 1L of rice wine is 994.374g. The temperature of the wine sample is 30°C, and the density of ethanol at this temperature is 0.781g / ml.

[0032] (2) Pretreatment of rice wine samples to be tested

[0033] Use a pipette gun to pipette 320ul of the rice wine sample to be tested into an empty common microplate, first add 40ul of a mixed aqueous solution of ...

Embodiment 3

[0041] Example 3 is the actual determination of the non-sugar solids of rice wine samples marked with an alcohol content of 13 by using the new method of the present invention.

[0042] Choose commercially available Tang and Song refined Huadiao wine (produced by Zhejiang Tang and Song Shaoxing Liquor Co., Ltd.), with an alcohol content of 13 degrees marked on the bottle. For the sake of accuracy, the alcohol content needs to be measured again, so based on the current national standard of rice wine, the alcohol content of this rice wine is determined to be 13.1 by the alcohol meter method. After weighing and conversion, the mass of 1L of rice wine is 1006.243g. The temperature of the wine sample is 30°C, and the density of ethanol at this temperature is 0.781g / ml.

[0043] Measured with reference to Example 2, the actual absorbance x=0.204±0.002, the relevant values ​​are shown in Table 3. If the total solids content measured by the national standard method is used as a benc...

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Abstract

A specific double-stranded DNA fragment has different degeneration degrees in ethanol-water systems with different mass-to-volume ratios of water and ethanol so as to cause difference of absorbance ina wavelength of 260nm, thereby establishing a brand-new determination method of non-sugar solids in the yellow rice wine with alcohol concentration of 12-14%. The specific double-stranded DNA fragment is shown in SEQ ID NO:1, and the relational expression between the absorbance x of the specific double-stranded DNA fragment and the mass-to-volume ratio y of water and ethanol in an ethanol-water system is as follows: y=-17.843x+10.067. The determination method of non-sugar solids is suitable for determination of the yellow rice wine with alcohol concentration of 12-14%, has good accuracy and reliability, is safer because of no need of long-time baking, is simple, convenient and rapid in operation, needs less wine sample amount, and is low in determination cost.

Description

technical field [0001] The invention relates to a method for detecting non-sugar solids, in particular to a simple and rapid method for detecting non-sugar solids in 12-14 degree yellow rice wine. Background technique [0002] Yellow rice wine is a special wine in my country. It has bright color, rich aroma, sweet taste, mellow flavor, and is rich in amino acids, small molecular sugars, organic acids, vitamins and trace elements. Rice wine is deeply loved by the masses of the people because of its rich nutrition, low alcohol content and unique flavor, mouthfeel and efficacy. Non-sugar solids are a general term for non-volatile components other than sugar in wine samples, and their proportion in rice wine is very small, usually less than 3%. Although the content is low, the non-sugar solids have a great effect. It determines the color, taste, nutrition and efficacy of rice wine to a large extent, and is an important index for the quality evaluation of rice wine. According t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N1/38
CPCG01N21/33G01N1/38
Inventor 陈荷冰陈如凯
Owner 陈荷冰
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