A small chemical derivative

Abstract

The invention discloses a small chemical derivation device. Derivation reaction tubes are pre-wound on a plurality of short heat-conducting rods to replace a traditional braided netted derivatizationdevice and the scheme of winding derivation reaction tubes onto U-shaped heat-conducting rods. The derivation reaction tubes are directly heated by the heat-conducting rods and heated by an air bath formed by an upper cover plate and a lower cover plate, so heating of the derivation reaction tubes is uniform and free of cold zones, and a temperature equalization error is + / - 0.2 DEG C. Short stainless steel pipes are used as liners of the ends of the derivation reaction tubes, stainless steel snapping sleeves and screwing caps are used out of the reaction tubes for sealing of the reaction tubes, and pressure-resistant sealing is more reliable, and a pressure as high as 4 MPa can be resisted. The chemical derivation device is applicable to on-line derivation of compounds like aflatoxin andallows the fluorescence intensity of aflatoxin B1 and G1 to be improved by 6.5 times. The whole chemical derivation device has better temperature equalization performance, high derivation efficiency,repeated and reliable performance, a small size, low power dissipation in heating and good practicality.

Description

technical field [0001] The invention relates to the technical field of high-performance liquid chromatography (HPLC) detectors, in particular to a small-scale chemical derivative for enhancing fluorescence detection signals. The derivative disclosed by the invention is matched with a fluorescence detector and can significantly improve the fluorescence response signals of the aflatoxins B1 and G1 when used to detect aflatoxins in food, medicinal materials and feed. Background technique [0002] Aflatoxin (AFTS) is a molecular mycotoxin, which is known as the strongest natural carcinogen. In 1993, aflatoxin was classified as a class I carcinogen by the Cancer Research Institute of the World Health Organization (WHO). Aflatoxins widely exist in moldy food products, and the detection of aflatoxins in agricultural products and food is an important international issue. At present, there are more than ten kinds of aflatoxins isolated, mainly including B1 (AFTB1), B2 (AFB2), G1 (AF...

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Problems solved by technology

Although the photochemical derivatization method is simple to operate, the excitation light source of the photochemical derivatizers currently on the market is a 254nm low-pressure mercury lamp, and its lifespan is only 2000-3000 hours. As the lighting time increases, its gradual aging will gradually reduce the light intensity. The long-term stability of the derivation results is not good; the iodine / bromine chemical derivatization method requires a post-column derivatization pump and preparation of a derivatization liquid, but its long-term derivation stability and repeatability are good, and it is also GB / T 18979-2003 food Test and derivation method specified in Chinese Pharmacopoeia 2015

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