A nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, its agent and its application

A technology of nitrogen-fixing bacteria and nitrogen, which is applied in the field of microorganisms and can solve problems such as limited applications

Active Publication Date: 2021-04-30
GUIZHOU EDUCATION UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the efficiency of symbiotic nitrogen fixation by rhizobia is higher than that of combined nitrogen fixation, it has a high degree of host specificity, which limits its application in non-host leguminous forages and non-legume forages

Method used

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  • A nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, its agent and its application
  • A nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, its agent and its application
  • A nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, its agent and its application

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Embodiment 1

[0064] Example 1, microbial nitrogen-fixing bacteria, whose classification is named as Microbacterium trichothecenolyticum SPJ01, is preserved in the China Center for Type Culture Collection, the strain preservation number is CCTCC NO: M2016254, the preservation date is May 9, 2016, and the preservation address is Wuhan, China. The microbial nitrogen-fixing bacterium provides nitrogen and phosphorus application to crops in soil, and relieves growth inhibition caused by nitrogen and phosphorus deficiency.

[0065] Microbial nitrogen-fixing bacteria were prepared as follows to provide nitrogen and phosphorus to crops: Microbacterium SPJ01 stored at 4°C was activated for 2 hours and then placed in a YEM liquid medium, shaken at 120r / min at 28°C Centrifuge at 10,000 r / min for 10 min until the absorbance at 665 nm of the bacterial solution is ≥0.8, wash the bacterial cells with sterile water, shake well and disperse to make OD 665 Bacterial suspension with a nm value of 0.5.

[00...

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Abstract

The invention discloses a microbial nitrogen-fixing bacteria, which is classified and named as Microbacterium trichothecenolyticum SPJ01, which is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC NO: M2016254, and the preservation date is May 9, 2016; the The application of the microbial nitrogen-fixing bacteria to provide nitrogen and phosphorus to crops in soil can alleviate the growth inhibition caused by the lack of nitrogen and phosphorus; the application of the microbial nitrogen-fixing bacteria as a nitrogen-fixing and growth-promoting bacterial agent in desert areas. It can not only alleviate the growth inhibition of crops under nitrogen and phosphorus deficiency stress, but also significantly promote the growth of crop seedlings, so that the growth indicators of the two forage grasses such as plant height, root length, single leaf area, single leaf The number, root volume, plant volume, aboveground fresh dry weight, underground fresh dry weight, plant fresh dry weight, root shoot ratio (R / T) and dry to fresh weight ratio (D / F) were significantly increased.

Description

technical field [0001] The invention relates to a nitrogen-fixing, phosphorus-decomposing, growth-promoting bacterium that can be used for desert plants, a bacterial agent and its application, and belongs to the technical field of microorganisms. Background technique [0002] As essential mineral elements for plants, nitrogen and phosphorus are important components of various structural and functional substances such as proteins, nucleic acids, phospholipids, enzymes, and plant hormones in plant living cells. It plays an important role in the process of gene expression and regulation and cell signal transduction. The lack of nitrogen and phosphorus will inhibit the growth and development of plants, cause some deficiencies, and even fail to complete their life cycle. Long-term large-scale application of chemical fertilizers not only increases the cost of agricultural production, but also causes the selective accumulation of certain elements in the soil and the deterioration ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 李剑峰张淑卿
Owner GUIZHOU EDUCATION UNIV
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