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Hemolytic agent used for detecting glycosylated hemoglobin and preparation method and application thereof

A technology of glycosylated hemoglobin and hemolytic agent, which is applied in the field of biochemistry, can solve the problems of increasing patient treatment costs and high detection costs, and achieve the effects of less environmental pollution, simple processing methods, and time saving

Inactive Publication Date: 2018-04-06
重庆艾维迪生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the current automatic glycosylated hemoglobin analyzers are all supporting reagents, and there is no good substitute for domestic reagents, resulting in high detection costs and increased treatment costs for patients.

Method used

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  • Hemolytic agent used for detecting glycosylated hemoglobin and preparation method and application thereof
  • Hemolytic agent used for detecting glycosylated hemoglobin and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 is used to detect the hemolyzing agent of glycated hemoglobin

[0048] Hemolyzing reagents used to detect glycated hemoglobin:

[0049] Dipotassium hydrogen phosphate: 10g / L;

[0050] Sodium chloride: 1g / L;

[0051] Phosphoric acid: 0.05g / L;

[0052] Triton: 1g / L;

[0053] Purified water is the solvent.

Embodiment 2

[0054] Embodiment 2 is used for detecting the hemolyzing agent of glycosylated hemoglobin

[0055] Hemolyzing reagents used to detect glycated hemoglobin:

[0056] Dipotassium hydrogen phosphate: 15g / L;

[0057] Sodium chloride: 2g / L;

[0058] Triton: 2g / L;

[0059] Phosphoric acid: 0.08g / L;

[0060] Purified water is the solvent.

Embodiment 3

[0089] The preparation method of embodiment 3 hemolytic agent

[0090] The preparation method of the hemolytic agent described in embodiment 1 and embodiment 2 may further comprise the steps:

[0091] 1) Take phosphate according to the above content, and add sodium chloride into the water medium to fully dissolve to obtain a mixed solution;

[0092] 2) Add phosphoric acid to the obtained mixed solution, then add purified water to fully dissolve it, prepare to a corresponding concentration, and then filter to obtain a hemolytic agent.

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Abstract

The invention belongs to the technical field of biochemistry, and concretely relates to a hemolytic agent used for detecting glycosylated hemoglobin and a preparation method and an application thereof. The hemolytic agent comprises a phosphatic buffer solution, sodium chloride, phosphoric acid and an aqueous medium; and content of the phosphatic buffer solution in the hemolytic agent is 6-30 g / L.According to the hemolytic agent, the detection accuracy after sample processing is high, the sample processing time is 10-30 s, and compared with same kind reagents, the processing time is short; thehemolytic agent can directly substitute an imported original product hemolytic agent, same detection capability with the imported reagent can be achieved, medical cost is reduced, and medical cost isreduced for patients.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and relates to a biochemical diagnostic reagent, in particular to a hemolyzing agent for detecting glycosylated hemoglobin and its preparation method and application. Background technique [0002] Glycated hemoglobin (HbA1c) is the product of the combination of hemoglobin and blood sugar in human blood. The combination of blood sugar and hemoglobin produces glycosylated hemoglobin, which is proportional to the blood sugar concentration and lasts for about 120 days. Therefore, monitoring glycosylated hemoglobin can reflect the blood sugar level of patients in 8-12 weeks. Changes. Therefore, it is used as an important indicator for the diagnosis and treatment of diabetes. sugar [0003] The higher the hemoglobin, the more blood sugar is combined with hemoglobin, and the diabetes is more serious. It avoids daily fluctuations in blood sugar levels, and has nothing to do with the patient's bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/72G01N35/00
CPCG01N33/723G01N35/00
Inventor 石功名杨凡
Owner 重庆艾维迪生物科技有限公司
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