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Natural medicine active component composition for preventing cerebral ischemia-reperfusion injury and preparation method and applications thereof

A technology of cerebral ischemia reperfusion and active ingredients, which is applied in the direction of drug combination, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of little knowledge about the pharmacological effects of the main active ingredients, and achieve promising development prospects, The effect of simple preparation method

Inactive Publication Date: 2018-04-10
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on its active ingredients, and little is known about the pharmacological effects of its main active ingredients

Method used

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  • Natural medicine active component composition for preventing cerebral ischemia-reperfusion injury and preparation method and applications thereof
  • Natural medicine active component composition for preventing cerebral ischemia-reperfusion injury and preparation method and applications thereof
  • Natural medicine active component composition for preventing cerebral ischemia-reperfusion injury and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Take blue cloth positive decoction pieces, add 10 times the amount of 70% volume concentration of ethanol, heat and reflux extraction 3 times, each time for 1.5 hours, filter, combine the filtrates, and concentrate the filtrate under reduced pressure to obtain the positive active ingredients of blue cloth. The yield is 10%-12%.

Embodiment 2

[0028] Example 2: Take blue cloth positive decoction pieces, add 10 times the amount of methanol, soak and extract at room temperature for 3 times, each time for 6 days, filter, combine the three filtrates, concentrate under reduced pressure, recover methanol, and obtain blue cloth positive active ingredients. The yield is 11%-14%.

Embodiment 3

[0029] Embodiment 3: Use LC-MS / QTRAP method to qualitatively detect the content of three active ingredients in the composition of blue cloth

[0030] Experimental method: In this experiment, 4500QTRAP was used for analysis in MIM-IDA-EPI mode. After ion screening in MIM mode, IDA triggered the linear ion trap for fragmentation. By analyzing the fragment ions and the structural characteristics of the three compounds, roughly Determine if three substances are present. The test results show that the composition is very likely to contain 3,4,5-trihydroxybenzaldehyde, temarin II, and casuarina tannin.

[0031] The experimental detection system is:

[0032] Liquid phase conditions - mobile phase A: deionized water; mobile phase B: 100% methanol; cleaning solution: 100% methanol; column temperature: 40 degrees; injection volume: 10uL, flow rate: 1.0mL / min; Gradient: 10% B;

[0033] Mass spectrometry conditions——MIN(MRM) are shown in Table 1:

[0034] Table 1

[0035]

[0036]...

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Abstract

The invention discloses a natural medicine active component composition for preventing cerebral ischemia-reperfusion injury and an extraction method and applications thereof. The natural medicine active component comprises 3,4,5-trihydroxyl benzaldehyde, a compound named Temalisu II, and casuarictin. The composition is prepared by the following steps: cutting whole herb of dry geum aleppicum, adding a methanol / ethanol solution, carrying out extraction for many times by adopting a reflux extraction or impregnation extraction mode, filtering the extracts, merging the extracts, carrying out vacuum concentration, and recovering methanol / ethanol to obtain the active component composition. The preparation method is simple, the active component composition can relieve cell apoptosis caused by cerebral ischemia-reperfusion injury and recover the cerebral functions, and the development prospect is good.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a natural medicine active ingredient composition for preventing and treating cerebral ischemia-reperfusion injury, a preparation method and application thereof. Background technique [0002] In recent years, stroke has become an important cause of human death and adult disability in the world, and acute ischemic stroke accounts for about 60%-80% of all stroke types. At present, restoring blood flow is the main method for the clinical treatment of acute ischemic stroke, such as rTPA thrombolytic therapy, which reduces the damage of the ischemic area by restoring the blood perfusion in the ischemic area; however, the sudden restoration of blood flow and May cause secondary damage to the ischemic area - ischemia-reperfusion injury. Ischemia-reperfusion injury is a common and complex pathophysiological process, which mainly leads to apoptosis. Apoptosis refers...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61K31/11A61K31/235A61K36/73A61P9/10
Inventor 梅志刚冯知涛陶薇黄亚光王凯欧炳金冯家腾杨彤吴韩钰张文丽
Owner CHINA THREE GORGES UNIV
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