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Extraction method of dendrobium officinale with antibacterial effect, and compound disinfecting and killing preparation

A technology of Dendrobium officinale and extract, which is applied in the directions of antifungal agents, preparations for skin care, biocides, etc., can solve the problems of few reports, low extraction yield of Dendrobium officinale polysaccharides, and high equipment cost

Pending Publication Date: 2021-09-14
宁波易中禾药用植物研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it has been reported that a single polysaccharide is used to prepare a disinfectant, but there are few reports on the preparation of a compound polysaccharide that enhances a more broad-spectrum antibacterial.
[0004] Generally speaking, the extraction yield of polysaccharides from Dendrobium officinale by the above-mentioned traditional techniques is still relatively low, while the emerging techniques have the disadvantage of degrading the polysaccharides due to the extraction time alone, destroying the structure of the polysaccharides, and reducing the activity of the polysaccharides. High cost and limitations

Method used

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  • Extraction method of dendrobium officinale with antibacterial effect, and compound disinfecting and killing preparation
  • Extraction method of dendrobium officinale with antibacterial effect, and compound disinfecting and killing preparation
  • Extraction method of dendrobium officinale with antibacterial effect, and compound disinfecting and killing preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Comparison of separate enzymatic hydrolysis extraction method experiments

[0037] Ref. Preparation of Dendrobium officinale extract. Take Dendrobium officinale, pulverize and pass through a 40-mesh sieve: add 4 times the amount of compound enzyme buffer (pectinase-cellulase-laccase (1-2:1-3:0.5-2.5), add glacial acetic acid solution to adjust the pH value to 4.0), control the enzymatic hydrolysis temperature at 50 °C for 140 min; add drinking water for 5 times, reflux for extraction for 2 h, filter (120 mesh sieve), concentrate the filtrate to a relative density of 1.30 (60 °C), add ethanol to make the solution alcoholic The amount reached 75%, stirred evenly, and after standing for 12 hours, collected the precipitate, evaporated the ethanol, and vacuum-dried at 70°C to obtain the obtained product. The results are shown in Table-1. Compared with the extract obtained by the above extraction method, the method of semi-biomimetic enzymolysis first and then ultr...

Embodiment 2

[0038] Embodiment 2 compares the experiment of ultrasonic extraction method alone

[0039] Ref. Preparation of Dendrobium officinale extract. Take Dendrobium officinale, vacuum dry it at 50°C for 2.5 hours, pulverize it through an 80-mesh sieve, add Dendrobium officinale powder with water at a solid-to-liquid ratio of 1:30, stir and mix, and place it in a stainless steel cylinder to keep it in a closed state. The container is added to the ultrasonic extractor for ultrasonic water bath heating extraction, the ultrasonic power is 300W, the ultrasonic frequency is 45Hz, the extraction temperature is 50-65°C, and the extraction time is 1h-1.5h. The suction filtration mixture is obtained; the suction filtration mixture is centrifuged at 5000-6000r / min for 10-15min to obtain the supernatant extract, concentrated, added with 95% ethanol, alcohol precipitation for 8h, centrifuged, and after the macroporous resin is oversized Dialysis. The results are shown in Table-1. Compared with ...

Embodiment 3

[0040] Example 3 Comparative ultrasonic-enzyme extraction method experiment

[0041] Ref. Preparation of Dendrobium officinale extract. Take Dendrobium officinale, dry at 65°C, pulverize 500 g of it, reflux 3 times with 15 times the weight of 95% ethanol, combine the dregs and dry, add distilled water according to the solid-liquid weight ratio of 1 / 25, ultrasonicate at 400W for 25 minutes, adjust the pH to 6.5, and use a water bath. 45° C., add 2.5% of cellulase and 2.5% of pectinase equivalent to the mass of the medicinal residue, enzymatically hydrolyze for 2.5 hours, and heat up to 55° C. in a water bath. Adjust pH to 6.8, add papain equivalent to 1.5% of the mass of the residue, enzymatically hydrolyze for 2.5h, heat at 92°C for 25min, centrifuge, filter, decolorize, concentrate, dialyze, add 95% ethanol, stand at 25°C for 18h, filter , to dry. The results are shown in Table-1. Compared with the extract obtained by the above extraction method, the method of semi-biomimet...

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Abstract

The invention discloses a preparation method for extracting a dendrobium officinale extract and a compound disinfecting and killing preparation by combining a semi-bionic enzyme and an ultrasonic method. The extraction method of the dendrobium officinale extract comprises the following steps: washing, drying, crushing, performing semi-bionic enzymolysis, performing semi-bionic ultrasonic treatment, decoloring, concentrating, washing and purifying. The semi-bionic enzyme-assisted extraction method is adopted, enzymolysis is conducted firstly, then semi-bionic ultrasonic extraction is conducted, extraction conditions are optimized, the dendrobium officinale extract is extracted, the whole technological process has the advantages of being free of pollutant emission, low in cost, short in production period, high in operability and high in yield, the method is suitable for large-scale production, the yield after semi-bionic enzymolysis is higher than that of conventional extraction, and development and utilization of dendrobium officinale resources are facilitated. The dendrobium officinale extract prepared by the method has better antibacterial and antiviral effects, and can be combined with other components to prepare spray, gel, ointment, hand sanitizer, wet tissue and other disinfection preparations.

Description

technical field [0001] The invention relates to a preparation method for extracting Dendrobium officinale extract and compound disinfecting preparation by combined use of semi-biomimetic enzyme-ultrasonic method, and belongs to the technical field of preparation of disinfecting products. Background technique [0002] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is the stem of Dendrobium officinale. , Coumarin, extracts, steroids and volatile oils. [0003] Dendrobium extract refers to extractive compounds obtained from Dendrobium plants. Modern research has shown that Dendrobium officinale polysaccharide has the functions of enhancing immunity, anti-oxidation, antibacterial, hypoglycemic, antihypertensive, and anti-tumor. Polysaccharides are natural biological macromolecules polymerized by monosaccharides, with complex structure, large molecular weight and high polarity. With the in-depth study of polysaccharides, more and more polysaccharid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/14C12P19/04A01N65/40A01N43/16A01N65/42A01P1/00A01P3/00A61K8/73A61K8/9789A61K8/9794A61P31/04A61P31/10A61P31/12A61Q17/00A61Q19/10
CPCC08B37/0003C12P19/14C12P19/04A01N65/00A01N65/40A01N43/16A01N65/42A61K8/9794A61K8/9789A61K8/73A61Q19/10A61Q17/005A61P31/04A61P31/10A61P31/12
Inventor 刘珺肖艺
Owner 宁波易中禾药用植物研究院有限公司
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