Preparation method of alkali-soluble polysaccharide of enteromorpha

An alkali-soluble and proliferative technology, which is applied in the field of preparation of prolifera alkali-soluble polysaccharide, can solve the problems of complicated steps, unsuitable for industrialized production, and high cost, and achieves high operability, is beneficial to development and utilization, and has low cost. Effect

Inactive Publication Date: 2018-11-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Claims
  • Application Information

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But the above-mentioned method or cost are too high, or steps are loaded down wi

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  • Preparation method of alkali-soluble polysaccharide of enteromorpha
  • Preparation method of alkali-soluble polysaccharide of enteromorpha
  • Preparation method of alkali-soluble polysaccharide of enteromorpha

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Embodiment 1

[0029] The preparation method of the enteromorpha alkali-soluble polysaccharide provided by the present embodiment comprises the following steps:

[0030] a. Wash the fresh Enteromorpha with water to remove the silt, and dry it at 45°C. The dried sample is pulverized by a pulverizer and passed through a 40-mesh sieve.

[0031] b. After mixing 100g of dry powder of Enteromorpha enteromorpha with 1000mL of 80% ethanol solution, ultrasonically treat at 60°C for 1 hour, filter and dry after the reaction.

[0032] c. Take the powder of Enteromorpha after heat reflux drying, heat reflux in 5000mL aqueous solution, react at 90°C for 2 hours, collect the residue, wash with hot water 3 times, and collect the residue.

[0033] d. Add the obtained residue to the compound enzyme solution (the ratio of cellulase and neutral protease is 1:1) at a solid-to-liquid ratio of 1:40 by weight, adjust the pH to 8.0, and react at 55°C for 80 minutes.

[0034] e. After the enzymatic hydrolysis react...

Embodiment 2

[0041] The preparation method of the enteromorpha alkali-soluble polysaccharide provided by the present embodiment comprises the following steps:

[0042] a. Wash the fresh Enteromorpha with water to remove the silt, and dry it at 45°C. The dried sample is pulverized by a pulverizer and passed through a 40-mesh sieve.

[0043] b. After mixing 50g of dry enteromorpha powder with 500mL of 80% ethanol solution, ultrasonically treat at 60°C for 1 hour, filter and dry after the reaction is completed.

[0044] c. Heat reflux dried Enteromorpha powder in 2500mL aqueous solution, react at 90°C for 2 hours, collect the residue, wash 3 times with hot water, and collect the residue.

[0045] d. Add the obtained residue to the compound enzyme solution (the ratio of cellulase and neutral protease is 1:1) at a solid-to-liquid ratio of 1:40 by weight, adjust the pH to 8.0, and react at 55°C for 80 minutes.

[0046] e. After the enzymatic hydrolysis reaction, centrifuge at 5000rpm for 10min to...

Embodiment 3

[0052] Embodiment 3 comparative experiment

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Abstract

The invention discloses a preparation method of alkali-soluble polysaccharide of enteromorpha. The preparation method comprises the following steps: a, washing the fresh enteromorpha with clean water,removing sediment, drying at the temperature of 45 DEG C, pulverizing the dried sample through a pulverizer, and screening through a 40-mesh sieve; b, processing through ultrasonic wave; c, thermallyrefluxing; d, conducting enzymolysis; e, extracting; f, concentrating, purifying and precipitating; g, washing; and h, purifying. The preparation method has the beneficial effects that the: no pollutant is discharged in the whole technological process, and the preparation method is suitable for large-scale production and is beneficial for the development and utilization of enteromorpha resources.The preparation method has the advantages of simple step, low cost, strong operability and high yield, and is easy to produce industrially. The extracting rate of the alkali-soluble polysaccharide ofenteromorpha is 16.38%, and is obviously increased compared with 13.42 reported in the literature.

Description

technical field [0001] The invention relates to a preparation method of enteromorpha alkali-soluble polysaccharide. The invention belongs to the field of marine microalgae cultivation. Background technique [0002] Enteromotpha, also known as moss strips, belongs to Chlorophyta (Chlorophyta), Ulvaceae (Ulvales), Ulvaceae (Ulvaceae) and the genus Enteromotpha. , Enteromorpha flat, Enteromorpha strips and Enteromorpha enterosum, etc. It is distributed in all sea areas in the north and south of my country, and belongs to the dominant species in the East China Sea. Enteromorpha is rich in nutrients, rich in carbohydrates and amino acids, and its iron content is the highest in my country's food. Enteromorpha has been used as edible and medicinal algae by coastal residents in my country since ancient times. In recent years, due to the large-scale reproduction of Enteromorpha, the ecological balance of the marine environment has been destroyed, and even serious "green tide" haza...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 刘楠周德庆孙永赵峰王珊珊
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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