EST-SSR marker for authenticating purity of favored hybrid seed of tomato rootstock varieties

A technology for hybrid seed purity and tomato, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve the problems of being easily affected by the environment, occupying a large area, and long identification period, so as to avoid identification The effect of long cycle, clear bands, and cost-saving identification

Inactive Publication Date: 2018-04-13
江苏绿港现代农业发展股份有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0006] In order to overcome the deficiencies of the traditional method for identification of tomato hybrids, such as long identification period, time-consuming and labor-intensive, large floor area, and easy to be affected by the environment, th...

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  • EST-SSR marker for authenticating purity of favored hybrid seed of tomato rootstock varieties
  • EST-SSR marker for authenticating purity of favored hybrid seed of tomato rootstock varieties

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Embodiment Construction

[0031] The present invention will be described in further detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto, and the protection scope of the present invention cannot be limited by the following examples.

[0032] The experimental methods used in the following implementation examples are conventional methods unless otherwise specified; the materials and reagents used, etc., can be obtained from commercial sources unless otherwise specified.

[0033] (1) Materials and methods

[0034] 1. Plant material

[0035] In this study, the seeds of tomato rootstock F1 variety Hobby and its female and male parents were used as experimental materials, and the seeds were sown and managed according to the experimental method of Jiangsu Lvgang Modern Agriculture Development Company (Lvgang for short).

[0036] 2. DNA extraction and detection

[0037] Tomato genomic DNA was extracted from fresh plant leaves about 21 days old by...

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Abstract

The invention discloses an EST-SSR marker for authenticating the purity of a favored hybrid seed of tomato rootstock varieties. A forward primer sequence of the EST-SSR marker is represented by SEQ IDNO.1, and a backward primer sequence of the EST-SSR marker is represented by SEQ ID NO.2. When the EST-SSR marker is utilized for carrying out PCR amplification, an about 126bp stripe is amplified from a favored female parent of the tomato rootstock varieties, an about 132bp stripe is amplified from a favored male parent of the tomato rootstock varieties, a 126bp/132bp hybrid stripe is amplifiedfrom a favored tomato rootstock variety F1, and the strips are clear, so that the favored hybrid seed of the tomato rootstock varieties can be differentiated from female parent seeds and male parent seeds, and the purity of the hybrid seed can be rapidly detected. According to the EST-SSR marker, the problems that a field cell authentication period is long, the time and the labor are wasted, the occupied area is large, and the like are avoided, the authentication time can be greatly shortened, and the authentication accuracy can be improved.

Description

technical field [0001] The invention relates to an EST-SSR marker, in particular to an EST-SSR marker for identifying the purity of tomato rootstock variety hobby hybrid seeds, and belongs to the field of biotechnology. Background technique [0002] tomato( Lycopersicon esculentum Mill.) has a large cultivation area. Because of its high yield, good benefit and rich nutritional value, it has become a popular vegetable variety and occupies a very important position in vegetable production. In recent years, the cultivation area of ​​protected tomatoes has been continuously expanding, and the production efficiency of tomato cultivation in protected areas has been greatly improved. However, due to the limited conditions of the facilities, crop rotation is difficult, and heavy cropping cultivation has caused serious pests and diseases, especially Fusarium wilt, bacterial wilt, and root-knot nematode. and other soil-borne diseases are increasing. The occurrence of soil-borne dis...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 卢霞阿门邓志军司龙亭
Owner 江苏绿港现代农业发展股份有限公司
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