SSR primer for identifying purity of seed of fruit CucumissativusL. Lvmei No.1 and method

Abstract

The invention discloses an SSR primer SSR17922 for identifying the purity of the seed of fruit CucumissativusL. Lvmei No.1. An upstream primer SSR17922-F is 5'-CATTCTAGGTCAATGAATCGCA-3', and a downstream primer SSR17922-R is 5'-GCAAAGTTGCCACATTGAAG-3'. Through the primers, the purity of the seed of the hybrid variety Lvmei No.1 can be accurately and quickly identified. Compared with traditional field plot identification, two specific clear banding patterns are amplified separately in a male parent and a female parent, a 182bp band is amplified in the female parent, a 197bp band is amplified inthe male parent, a 182 / 197bp heterozygous band simultaneously containing the specific banding patterns of the male parent and the female parent is amplified in F1 variety Lvmei No.1, the band is clear, the hereditary is stable, and the fruit CucumissativusL. Lvmei No.1 can be distinguished from the seed of the male parent, the seed of the female parent and other hybrid seeds, so that the purity of the seed of the hybrid variety Lvmei No.1 can be quickly detected. The shortcomings of long period, time-consuming, labor-intensive, and large occupied area of the traditional field plot identification are overcome, a traditional hybrid seed purity identification method can be replaced, the identification time is greatly shortened, the identification cost is saved, the identification accuracy isimproved, and the commercial application value is high.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an SSR marker used for the purity identification of the seeds of the fruit cucumber hybrid "Lvmei No. 1". Background technique [0002] cucumber( Cucumissativus L . ) is Cucurbitaceae ( Cucurbitaceae ) Melon ( Cucumis L . ) is an annual herbaceous climbing plant, which has been cultivated in my country for more than 2,000 years. It is one of the main vegetable crops in the world and plays an important role in vegetable production. Cucumber has strong heterosis, and the first-generation hybrid seeds are widely used in the market. Cucumber breeders have bred many high-yield, high-quality, and disease-resistant varieties, which have made great contributions to increasing and stabilizing agricultural production. However, due to seed production technology, false hybrids formed by mechanical mixing, and fake and shoddy seeds purchased and sold by some unscrupulous trade...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the shortcomings of current cucumber hybrid seed purity identification based on field phenotype observation methods such as large environmental impact and long identification time, and the purpose of the present invention is to provide an SSR primer for identifying the purity of cucumber hybrid Lvmei No. 1 seeds , the primers can produce specific markers in the corresponding parental parents, with strong specificity, and can quickly, accurately and efficiently identify the purity of the seeds of the cucumber hybrid Lumei No. 1

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