Detection method of traditional Chinese medicine composition capable of invigorating brain and tonifying kidney

A detection method and composition technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot fully reflect the components of traditional Chinese medicine compound, and achieve the effect of comprehensive detection characteristics, simple technical operation, and good repeatability

Inactive Publication Date: 2018-05-08
王荔强
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Problems solved by technology

[0005] Aiming at the problem that the current quality detection technology of brain-tonifying and kidney-tonifying traditional Chinese medicine composition is simple and cannot fully reflect the components contained in the compound Chinese medicine, the purpose of the present invention is to establish an HPLC characteristic spectrum detection technology for the brain-tonifying and kidney-tonifying traditional Chinese medicine composition, and to carry out the detection of its drug components. effective detection

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  • Detection method of traditional Chinese medicine composition capable of invigorating brain and tonifying kidney
  • Detection method of traditional Chinese medicine composition capable of invigorating brain and tonifying kidney
  • Detection method of traditional Chinese medicine composition capable of invigorating brain and tonifying kidney

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Embodiment Construction

[0024] The following examples are given to further describe the present invention in detail, and these examples are only used to illustrate the present invention and do not limit the present invention.

[0025] 1. Chromatographic conditions and system suitability test

[0026] Instruments: American Diana high performance liquid chromatography (U3000 pump, UVD170U ultraviolet detector, CHROMELEON data processing software, manual injector, column thermostat);

[0027] Chromatographic column: Kromasil-C18 chromatographic column (250×4.6mm);

[0028] Balance: startorius BP211D electronic analytical balance (d=0.01mg);

[0029] Vacuum degassing system;

[0030] National Pharmacopoeia Commission Chinese medicine chromatographic feature map similarity evaluation system;

[0031] Mobile phase: Acetonitrile was used as mobile phase A, 0.1% phosphoric acid solution was used as mobile phase B, and gradient elution was carried out according to the provisions in Table 1;

[0032] Detec...

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Abstract

The invention discloses a detection method of a traditional Chinese medicine composition capable of invigorating brain and tonifying kidney, and belongs to the technical field of traditional Chinese medicine quality detection. According to the detection method, high performance liquid chromatography is adopted, under the same chromatographic conditions, paeoniflorin is taken as a reference, and characteristic qualitative analysis on chlorogenic acid, spinosin, forsythin, and arctiin in samples is carried out. The detection method is simple and rapid, and is high in sensitivity and stability; acomposition characteristic spectrum is obtained; and comprehensive detection of all contents of the composition is realized.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, and relates to a quality detection method of a brain-tonifying and kidney-tonifying traditional Chinese medicine composition, and specifically belongs to the technical field of quality detection of traditional Chinese medicine preparations. Background technique [0002] Jiannao Bushen Wan is an old variety of Chinese patent medicine that has been sold publicly since the 1950s. Composed of flavored traditional Chinese medicine, it has the functions of strengthening the brain and kidney, replenishing qi and invigorating the spleen, calming the nerves and calming the mind. For amnesia, insomnia, dizziness, tinnitus, palpitations, soreness of the waist and knees, nocturnal emission caused by spleen and kidney deficiency; neurasthenia and sexual dysfunction see the above symptoms. [0003] Because this product is a large prescription of 25 herbs, the ingredients are complex. At present, t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王荔强郭桂秋翟勇袁新秀宁乐
Owner 王荔强
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