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Method for extracting long-chain dibasic acid from fermentation broth

A technology of long-chain dibasic acid and dibasic acid, which is applied in the field of C10~C18 dibasic acid extraction, can solve the problems of ineffective removal of protein, high total nitrogen content, affecting product purity, etc.

Active Publication Date: 2018-05-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is to add a salting-out agent to the system, which will reduce the solubility of the protein and precipitate out of the solution with the long-chain dibasic acid salt, and there is no effective method for removing the protein in the subsequent steps, so that the protein cannot be effectively removed. In addition, thus affecting the purity of the product, the total nitrogen content is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The filter cake obtained in Comparative Example 1 was mixed with 90 mL of deionized water for beating, and pyridine was added thereto to adjust the pH value to 5.4, and the reaction was carried out for 60 min under stirring. Then carry out membrane filtration through an ultrafiltration device with a molecular weight cut off of 5000 to obtain a filtrate. Sodium hydroxide was added to the filtrate to adjust the pH value to 6.5 to form long-chain dibasic acid monosodium salt, which was crystallized, filtered, and washed with 10°C low-temperature deionized water to obtain long-chain dibasic acid monosodium salt crystals. Mix the crystals with fresh deionized water and heat to 95°C, add 2 g of activated carbon for adsorption, then filter, add H to the filtrate 2 SO 4 The pH of the filtrate was adjusted to 3.0 to obtain C 12 h 22 o 4 An aqueous solution of crystallization, filtered, and washed with water until neutral to obtain a wet cake, which was then dried to obtain C...

Embodiment 2

[0040] Take 200mL to obtain C by Candida tropicalis fermentation 13 h 24 o 4 Fermentation liquid, fermented liquid with a concentration of 154g / L, heated at 85°C, filtered through a membrane to obtain a filtrate, and used H 2 SO 4 The pH of the filtrate was adjusted to 3.0 to obtain C 13 h 24 o 4 The aqueous solution of crystallization was filtered and washed with water until neutral to obtain a wet filter cake.

[0041] The filter cake was mixed with 60 mL of deionized water for beating, 3-picoline was added thereto to adjust the pH to 5.6, and the reaction was carried out for 60 min under stirring. Then perform membrane filtration through an ultrafiltration device with a molecular weight cut-off of 30,000 to obtain a filtrate. Sodium hydroxide was added to the filtrate to adjust the pH value to 7.0 to form long-chain dibasic acid monosodium salt, which was crystallized, filtered, and washed with 10°C low-temperature deionized water to obtain long-chain dibasic acid mo...

Embodiment 3

[0044] Take 200mL to obtain C by Candida tropicalis fermentation 14 h 26 o 4 Fermentation broth, the fermentation broth with a concentration of 176g / L, is heated to 80°C, filtered through a ceramic microfiltration membrane, and bacteria and residual liquid wax are removed to obtain a long-chain dibasic acid salt filtrate. Add H to the filtrate 2 SO 4 Adjust the pH to 2.4 to obtain C 14 h 26 o 4 The aqueous solution of crystallization was filtered and washed with water until neutral to obtain a wet filter cake.

[0045] The filter cake was mixed with 90 mL of deionized water for beating, 2,4-lutidine was added thereto to adjust the pH value to 5.4, and the mixture was reacted for 60 min under stirring conditions. Then perform membrane filtration through an ultrafiltration device with a molecular weight cut-off of 1000 to obtain a filtrate. Sodium hydroxide was added to the filtrate to adjust the pH value to 6.8 to form long-chain dibasic acid monosodium salt, which was ...

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PUM

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Abstract

The invention discloses a method for extracting long chain dibasic acid from fermentation broth. The method comprises the following steps: acidifying and crystallizing pretreated termination fermentation broth, mixing with water, pulping, adding a pyridine compound, filtering through a membrane, adding a sodium hydroxide solution to filtrate, mixing crystals obtained by the filtration with water,heating, dissolving, adsorbing, filtering, and adding an acid to the filtrate for acidification and crystallization to obtain the long-chain dibasic acid product. The long-chain dibasic acid extractedby the method has the advantages of high purity, white color, low protein content, loose and easy drying of the product, and the like.

Description

technical field [0001] The present invention relates to a C 10 ~C 18 Methods of dibasic acid extraction, especially from C 10 ~C 18 A method for extracting long-chain dibasic acids from n-paraffin fermentation broth. Background technique [0002] The long-chain dibasic acid molecule general formula that the present invention refers to is C n h 2n-2 o 4 , where n is 10-18, is a metabolite obtained by microorganisms using alkanes to ferment, and exists in the fermentation broth as a long-chain dibasic acid in the form of a double salt. [0003] Long-chain dibasic acids are metabolites obtained from the fermentation of normal alkanes by microorganisms. Its fermentation broth is a complex multiphase system, which contains unreacted normal alkanes, microbial cells and debris, unused medium and metabolites, and microbial secretions, etc., especially containing a large amount of impurities such as proteins and pigments , affecting the purity and appearance quality of the pr...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/02C07C55/21C07C55/02
CPCC07C51/02C07C51/412C07C51/42C07C55/21C07C55/02
Inventor 高大成李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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