Method for evaluating bitterness of fructus citrus sarcodactylis juice used for making beer
A technology of bitterness and bergamot, which is applied in the field of beer quality evaluation, can solve the problems that there is no effective evaluation method for the bitterness of bergamot juice
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Embodiment 1
[0011] Example 1 Correlation analysis between limonin content and standard bitter solution
[0012] The configuration of quinine standard solution: take distilled water as the dissolution medium, and configure the concentration successively to be 1c (c=3×10 -6 mol / L), quinine standard solutions of 2c, 4c, 8c, 12c, 16c, 20c, 24c, 28c, 32c, set aside.
[0013] Configuration of limonin series solutions (v / v): Take bergamot juice with a purity of 100% (after washing fresh bergamot, put it directly into a juicer to extract the juice, centrifuge at 3000r / min and collect the supernatant for later use), and then Bergamot juice samples were diluted with distilled water to concentrations of 10% (a1), 20% (a2), 40% (a3), 60% (a4), 80% (a5), and 100% (a6).
[0014] (1) Evaluation of the bergamot juice solution: After rinsing the mouth with distilled water, the evaluators took 2-3 mL of the prepared bergamot juice solution (a1-a6) to be evaluated, put it in the mouth, and spit it out afte...
Embodiment 2
[0024] Correlation analysis between embodiment 2 hop extract solution and standard bitter solution
[0025] Configuration of hop extraction solution (g / 100mL): use distilled water as the extraction medium, add concentrations of 0.01% (b1), 0.03% (b2), 0.075% (b3), 0.15% (b4), 0.3% ( The hops of b5) are boiled for 45 minutes.
[0026] (1) Evaluation of the hop extraction solution: After rinsing the mouth with distilled water, the assessor takes 2-3 mL of the hop extraction solution (b1~b5) to be evaluated, puts it in the mouth, and spits it out after 10 seconds. After rinsing your mouth with distilled water, take a quinine standard solution with a similar taste and taste it. If you confirm that the two tastes are similar, you can determine the bitterness value of the liquid to be evaluated as the bitterness value of the quinine standard solution, otherwise you need to use other standards Taste the liquid again until the bitterness value is determined. Rinse your mouth before ...
Embodiment 3
[0035] Embodiment 3 Establishment of the evaluation method for the bitterness of bergamot juice
[0036] With the standard bitter substance quinine as the medium, the conversion relationship between the limonin content (equation 1) and the beer bitterness value (equation 2) is established: y=e 3.8878lnx+0.4172 (Equation 3), wherein, x is the limonin content, and y is the hop bitterness value. The bitterness of bergamot juice / bergamot beer can be evaluated by Equation 3 based on the determination of limonin content.
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