Automatic focusing method and system

An auto-focus and best-focus technology, applied in the field of auto-focus methods and systems, can solve problems such as low efficiency, auto-focus methods and complex systems

Inactive Publication Date: 2018-05-11
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide an automatic focusing method and system, aiming to solve the

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  • Automatic focusing method and system
  • Automatic focusing method and system

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Embodiment Construction

[0099] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0100] The present invention proposes a first embodiment. Such as figure 1 Shown, a kind of automatic focusing method is characterized in that, described method comprises the following steps:

[0101] S01, the photographing step, controlling the imaging device to photograph the sequencing reaction region at different focus positions, and obtaining multiple sequencing images;

[0102] S02. The step of obtaining the best focus position, using the magnetic bead identification threshold to identify the area values ​​of all the magnetic beads in the first regions of the plurality of sequencing images, based on the area values ​​of all the magnetic beads in the first regions of the plurality of sequencing images The area value of the bead determines th...

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Abstract

The invention relates to the field of image shooting, and provides an automatic focusing method and system. The automatic focusing method comprises the steps of shooting, that is, controlling an imaging device to shoot a sequencing reaction region at different focusing positions to obtain a plurality of sequencing images; acquiring the optimum focusing position, that is, respectively recognizing an area value of all magnetic beads in a first region of the plurality of sequencing images by using a magnetic bead recognition threshold, and determining the optimum focusing position based on the area value of all magnetic beads in the first region of the plurality of sequencing images; and focusing, that is, moving the imaging device to the optimum focusing position. Automatic focusing can be realized quickly and efficiently according to the method and system provided by the invention.

Description

technical field [0001] The present invention relates to the field of image capture, and more specifically, to an automatic focusing method and system. Background technique [0002] In the prior art, the shooting module is often moved with a fixed step length to take pictures of the subject, and then obtain a series of images with different focal lengths, and then obtain the image with the best definition by analyzing the sharpness values ​​of these images, and define the The position corresponding to the image with the best definition is the best focus position, and then the shooting module is moved to the best focus position for shooting. However, when the above method is applied to the field of high-throughput gene sequencing, the method for judging the clarity value of the sequencing image is relatively complicated and the efficiency is low. [0003] Therefore, there is a need for a new method and system capable of quickly realizing automatic focusing. Contents of the ...

Claims

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Application Information

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IPC IPC(8): G02B7/38G03B13/36
CPCG02B7/38G03B13/36
Inventor 盛司潼冀高
Owner GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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