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Biological marker of epithelial ovarian cancer and application of biological marker

A technology for ovarian cancer and epithelium, which is applied in the field of molecular biology and the preparation of disease diagnostic reagents, and can solve the problems of unknown functional significance of risk variables, etc.

Inactive Publication Date: 2018-05-29
SDIVF R&D CENT LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional significance of these risk variables is largely unknown

Method used

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  • Biological marker of epithelial ovarian cancer and application of biological marker
  • Biological marker of epithelial ovarian cancer and application of biological marker
  • Biological marker of epithelial ovarian cancer and application of biological marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 patient and sample

[0042] The subjects were 74 patients with familial epithelial ovarian cancer and 47 patients with non-epithelial ovarian cancer (controls). miRNA and mRNA were obtained from lymphocytes in the peripheral blood of the subjects. There were no familial relationships among the 74 patients with familial epithelial ovarian cancer. In the families of these patients, at least two first- or second-degree relatives were diagnosed with genetic ovarian cancer at a certain age. None of these patients had BRCA1 / 2 or MLH1 / MSH2 mutations.

Embodiment 2

[0043] Example 2 Analysis of the target gene relationship and action pathway of epithelial ovarian cancer-related microRNAs and mRNAs studied in the present invention

[0044] Using the algorithm shown in Table 1, namely Miranda (microRNA.org), microcode (http: / / www.mircode.org / mircode / ), MirTarget2[21], Targetscan (http: / / www.targetscan.org / ), the microRNAs involved in the method of the present invention, especially the influence factors (microRNA and mRNA) and the action relationship of the core microRNAs (hsa-miR-34b, Hsa-miR-335, hsa-miR-136) are analyzed.

[0045] The results are shown in Table 1. In the samples of epithelial ovarian cancer patients, 16 interacting microRNA and mRNA pairs were found; the involved microRNAs include: hsa-miR-34b, hsa-miR-335, hsa-miR-136, hsa-let-7e, hsa-miR-1297, hsa-miR-374b and hsa-miR-891b; the genes involved include TAOK1, MGEA5, ZBED5, MMP3, DNAJA1, KLHL28, PDCD7, LSM12, SLC9A6, ATG7, C1orf63. In samples from patients with epithelia...

Embodiment 3

[0056] Example 3 gene chip analysis miRNA expression and mRNA expression

[0057] Using the miRNA expression data (data number GSE31801) obtained by the Illumina Human v2 MicroRNA expression beadchip, the gene expression comprehensive database (Gene expression omnibus, GEO) published in: http: / / www.ncbi.nlm.nih.gov / geo / queryacc.cgi?acc=GSE31801) for analysis. The miRNA plate of the chip includes 1146 human miRNAs (>97% miRNAs disclosed in miRBase release 12) to analyze the expression of miRNAs in the aforementioned subjects. Divide the miRNA analysis area of ​​the chip into 3 areas. Among them, the detection of the first region includes hsa-miR-34b, hsa-miR-335, hsa-miR-136; the second region includes hsa-let-7e, hsa-miR-1297, hsa-miR-374b and hsa-miR -891b; the third region includes the remaining miRNAs.

[0058] In addition, the mRNA expression data obtained by the Illumina expression detection chip (llumina HumanHT-12 V3.0 expression beadchip) (data number GSE37582: http...

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Abstract

The invention provides a biological marker of epithelial ovarian cancer and application of the biological marker. The invention provides a method for researching and detecting the epithelial ovarian cancer by the combination of the following miRNA: hsa-miR-34b, hsa-miR-335, hsa-miR-136, hsa-let-7e, hsa-miR-1297, hsa-miR-374b and hsa-miR-891b. The invention also provides a kit and a device for themethod for researching and detecting the epithelial ovarian cancer.

Description

technical field [0001] The invention relates to the fields of molecular biology and preparation of disease diagnosis reagents. Specifically, the present invention relates to biomarkers of miRNA and mRNA groups of epithelial ovarian cancer and methods for studying epithelial ovarian cancer, and the use of said markers in the preparation of reagents for diagnosing or detecting epithelial ovarian cancer box application. Background technique [0002] Epithelial ovarian cancer (EOC) is the most common gynecological malignancy in women, accounting for nearly 90% of ovarian malignancies [1, 2]. At diagnosis, most patients (75%) had advanced disease and almost all experienced tumor recurrence [3,4]. [0003] About 10% of ovarian cancer cases are due to familial / genetic predisposition, mainly due to mutations in mismatch repair genes in BRCA1 and BRCA2, but the genetic mechanism still needs further study. Several genome-wide association studies (GWAS) have been performed in ovaria...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 刘洪彬马金龙耿玲苏献伟熊志强路钢
Owner SDIVF R&D CENT LTD
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