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Lung cancer screening kit

A kit and reagent technology, applied in the field of lung cancer screening kits, can solve the problems of no ZAP70, no expression of phosphorylated ZAP70, etc., and achieve good clinical application prospects

Active Publication Date: 2018-05-29
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on ZAP70 is mostly focused on its signal transduction related to chronic B lymphocytic (B-CLL), and there is no report on the relationship between ZAP70 and lung cancer, and there is no report on the expression of phosphorylated ZAP70 in lung cancer

Method used

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  • Lung cancer screening kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The relationship between the expression level of embodiment 1p-ZAP70 and lung cancer

[0021] 1. Experimental method

[0022] 1. Clinical data

[0023] 20 cases of paraffin sections of lung cancer patients and 20 cases of remote controls (referring to paraffin sections of distal normal lung tissue of lung cancer patients, 5 cm away from cancer tissue) were selected. The basic information is shown in Table 1.

[0024] Table 1 Basic clinical information

[0025]

[0026] * Note: Others include large cell carcinoma, adenosquamous carcinoma, and small cell carcinoma.

[0027] 2. Detection of p-ZAP70 expression level

[0028] Immunohistochemistry (IHC) using p-ZAP70 as an index was performed on paraffin sections of lung cancer patients and control paraffin sections of distant tissues.

[0029] p-ZAP70 antibody: purchased from Affinity Biosciences, Cat. No. AF3313;

[0030] EnVision TM HRP secondary antibody, antibody diluent, DAB chromogenic reagent, antigen retri...

Embodiment 2

[0059] Example 2 The present invention detects the composition of the kit for the expression level of p-ZAP70 and its application method

[0060] 1. The composition of the immunohistochemical detection kit

[0061] Detection kit (50 copies):

[0062] components

volume

p-ZAP70 antibody

50μl

EnVision TM HRP secondary antibody

5ml

Antibody Diluent

5ml

DAB developer

5ml

Antigen Retrieval Solution

500ml

Wash Buffer *

1000ml

immunohistochemistry pen

1 stick

[0063] Note: This kit is calculated as 100 μl of various experimental reagents per slice, and the specific dosage can be appropriately increased or decreased according to the size of the tissue.

[0064] 2. How to use the kit

[0065] The lung tissue of the sample to be tested was prepared as a paraffin section as a test specimen, and the expression level of p-ZAP70 was detected according to the following method:

[0066] (1) Block...

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PUM

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Abstract

The present invention discloses a lung cancer screening kit comprising an optional reagent for detecting the expression level of p-ZAP70. The present invention also discloses use of the reagent for detecting the expression level of the p-ZAP70 in the preparation of a reagent for screening for lung cancer. The kit can determine the risk of lung cancer among subjects by detecting the expression level of the pZAP70, can be used for auxiliary diagnosis of clinical lung cancer, provides patients an effective basis for relevant therapeutic measures or decisions, and has good clinical application prospects.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/573
CPCG01N33/573G01N33/57423G01N2333/91215
Inventor 陈菲李丽陈红英步宏张立
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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