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A method for extracting extracellular polymers from mycolloids

A technology of extracellular polymers and bacterial micelles, applied in the field of bioengineering, can solve problems such as cell rupture, and achieve the effects of avoiding inactivation, efficient separation, and convenient research and measurement

Active Publication Date: 2021-01-05
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no single method for extracting all EPS from a sample, all methods cause cell disruption to some degree

Method used

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  • A method for extracting extracellular polymers from mycolloids
  • A method for extracting extracellular polymers from mycolloids
  • A method for extracting extracellular polymers from mycolloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The extracellular polymer of a black anaerobic granular sludge was extracted by the method described in this patent and its composition was determined. The specific operation steps are as follows:

[0040] (1) Obtain a sludge sample from the reactor and measure its volume to be 30mL. Wet the filter paper and cover it on the Buchner funnel, place the sludge on the filter paper, turn on the suction filter, and adjust the pressure of the suction filter to 0.03MPa. Slowly pour 0.8% sodium chloride solution into the funnel to clean the sludge.

[0041] (2) Take out the treated sludge, put it into a beaker, and add sodium chloride solution to restore the volume to 30mL. Divide into 1.5mL centrifuge tubes, put them into a centrifuge, adjust the centrifuge speed to 600r / min, set the time to 15min, and extract the supernatant as a mucus layer.

[0042] (3) Take out the sludge in the centrifuge tube, and use sodium chloride solution to restore the sludge volume to 30mL. Sludg...

Embodiment 2

[0047] The method described in this patent was used to extract the extracellular polymer of a certain red anammox granular sludge and determine its composition. The specific operation steps are as follows:

[0048] (1) The sludge sample was obtained from the anammox reactor, and its volume was determined to be 30mL. Wet the filter paper and cover it on the Buchner funnel, place the sludge on the filter paper, turn on the suction filter, and adjust the pressure of the suction filter to 0.03MPa. Slowly pour 0.8% sodium chloride solution into the funnel to clean the sludge.

[0049] (2) Take out the treated sludge, put it into a beaker, and add sodium chloride solution to restore the volume to 30mL. Divide into 1.5mL centrifuge tubes, put them into a centrifuge, adjust the centrifuge speed to 600r / min, set the time to 15min, and extract the supernatant as a mucus layer.

[0050] (3) Take out the sludge in the centrifuge tube, and use sodium chloride solution to restore the slu...

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Abstract

The invention provides a method for efficiently extracting an extracellular polymeric substance of activated sludge. The method comprises cleaning, extracting a slime layer by a centrifugal method, extracting a loosely bound extracellular polymeric substance (LB-EPS) by a high-speed centrifugal method and extracting a tightly bound extracellular polymeric substance (TB-EPS) by a formaldehyde-acetic acid potassium acetate heating method. The method has the beneficial effects that the method provided by the invention can be used for simply, conveniently and quickly extracting the extracellular polymeric substance of the activated sludge, moreover, can be used for avoiding the decomposition of protein and polysaccharide, which is caused when a cellular content is dissolved out into an extracting solution and is extracted, and the purity of an extract is furthest guaranteed.

Description

technical field [0001] The invention relates to a method for extracting extracellular polymers from activated sludge fungal micelles, which can be applied to the extraction and research on properties of activated sludge extracellular polymers. Specifically, the invention can be applied to sewage treatment and belongs to the technical field of bioengineering. Background technique [0002] Extracellular Polymeric Substances (EPS) is a general term for extracellular polymer substances secreted by microbial cells during metabolism, mainly including polysaccharides, nucleic acids and lipids. Extracellular polymer is an important component of activated sludge. It has a certain degree of hydrophobicity and can combine with cations, which has an important impact on the surface charge, sedimentation, flocculation and dehydration of sludge. To study the influence of extracellular polymers on sludge properties and its quantitative analysis, a method suitable for extracting extracellul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12N15/10C08B37/00
CPCC07K1/14C08B37/0003C12N15/1003
Inventor 姬丹丹臧立华毛家明
Owner QILU UNIV OF TECH
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