Marine bacterium and application thereof
A marine bacteria, nano-palladium technology, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problem of no marine bacteria, etc., and achieve the effect of strong stress resistance
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Embodiment 1
[0036] Take 0.1g of the sediment obtained in the Bohai Strait (N 38° 30.29', E 121° 14.10') and inoculate it into LB medium (10g / L peptone, 5g / L yeast extract, 10g / L sodium chloride) Cultivate overnight at 37°C on a shaker (150rpM), and screen multiple strains of pure bacteria by the plate coating method. Further use the inorganic salt medium containing Au(III) as the test system to screen for the ability to reduce Pd(II) to Pd 0 (Apparently it can change from earthy yellow to black), reducing Au(III) to Au 0 (Appearance can change from colorless to pink or purple) strains for follow-up studies.
[0037] Based on the above background, the present invention uses the marine bottom mud in the middle of the Bohai Strait as the bacterial source, and screens out a marine indigenous bacterium with precious metal gold and palladium reducing ability, which is named GP, and is identified as Bacillus sp., It has been preserved in the China Center for Typical Culture Collection, address...
Embodiment 2
[0039] Mix the above-mentioned obtained bacterial strain GP (0.5g / L) and Pd(II) (50mg / L) in 100mL inorganic salt medium, the salinity is 2%, and the pH is 7.2, then add 2g / L of sodium lactate, expose Nitrogen for 10 minutes, and react in the dark for 36 hours in an incubator at 30°C. The resulting mixture is shown in figure 1 a. As shown in the figure, after 36 hours of reaction, the color of the solution changed from earthy yellow to brownish black, presumably due to the change of Pd(II) to Pd 0 , where the blank system is a bacterial suspension.
[0040] Mix the above-mentioned obtained bacterial strain GP (0.5g / L) and Au(III) (50mg / L) in 100mL inorganic salt medium, the salinity is 2%, and the pH is 7.2, then add 2g / L of sodium lactate, expose Nitrogen for 10 minutes, and reacted in a 30°C incubator in the dark for 48 hours. The resulting mixture is shown in figure 1 b. As shown in the figure, after 48 hours of reaction, the color of the solution changed from colorless ...
Embodiment 3
[0042] Preparation of bionano palladium:
[0043] Mix the above-mentioned obtained bacterial strain GP (0.5g / L) and Pd(II) (50mg / L) in 100mL inorganic salt medium, the salinity is 2%, and the pH is 7.2, then add 2g / L of sodium lactate, expose Nitrogen for 10 min, and react in the dark for 36 h in an incubator at 30 °C, and the resulting mixture ( figure 1 A) Centrifuge (10000rpm, 5min), put the precipitate in deionized water and ultrasonically treat it for 1h, centrifuge again and dry the precipitate in an oven at 60°C, and the powder obtained by X-ray diffraction energy spectroscopy is the prepared bionano palladium ( Figure 2A ).
[0044] Inorganic salt medium is per liter, 1g NH 4 Cl, 0.8g Na 2 HPO 4 ,0.2g KH 2 PO 4 ,0.2g MgCl 2 ·7H 2 O, 0.1g CaCl 2 2H 2 O, 20g NaCl, the balance is deionized water. , the reaction temperature, salinity and pH were 30°C, 2% and 7.2, respectively.
[0045] From the XRD standard spectrum (JCPDS 46-1043) of palladium described in t...
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