A kind of marine bacteria bacillus sp. GP and its application
A technology of marine bacteria and precious metals, applied in the direction of bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problems of no marine bacteria and achieve strong stress resistance
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Embodiment 1
[0036] Take 0.1 g in the Bohai Strait (N 38 o 30.29', E 121 o 14.10’) obtained sediment, inoculated into LB medium (10 g / L peptone, 5 g / L yeast extract, 10 g / L sodium chloride) on a shaker (150 rpM) for 37 o C was cultured overnight, and multiple strains of pure bacteria were screened by the plate coating method. Further, the inorganic salt medium containing Au(III) was used as a test system to screen for the ability to reduce Pd(II) to Pd0 (apparently it can change from earthy yellow to black), and to reduce Au(III) to Au0 (apparently Strains that can change from colorless to pink or purple) are used for follow-up studies.
[0037] Based on the above background, the present invention uses the marine bottom mud in the middle of the Bohai Strait as the source of bacteria, and screens out a marine indigenous bacterium with precious metal gold and palladium reducing ability, which is named GP, and is identified as Bacillus sp., It has been preserved in the China Center for Typ...
Embodiment 2
[0039] Mix the above obtained strains GP (0.5 g / L) and Pd(II) (50 mg / L) in 100 mL of inorganic salt medium with a salinity of 2% and a pH of 7.2, then add 2 g / L of sodium lactate , nitrogen exposure for 10 min, at 30 o In the incubator of C, the reaction was protected from light for 36 h, and the resulting mixture was shown in figure 1 a. As shown in the figure, after 36 hours of reaction, the color of the solution changed from earthy yellow to brownish black, presumably because Pd(II) changed to Pd0, and the blank system was bacterial suspension.
[0040] Mix the above obtained strains GP (0.5 g / L) and Au(III) (50 mg / L) in 100 mL inorganic salt medium with a salinity of 2% and a pH of 7.2, then add 2 g / L of sodium lactate , nitrogen exposure for 10 min, at 30 o In the incubator of C, the reaction was protected from light for 48 h, and the obtained mixture was shown in figure 1 b. As shown in the figure, after 48 hours of reaction, the color of the solution changed from c...
Embodiment 3
[0042] Preparation of bionano palladium:
[0043] Mix the above-mentioned obtained strains GP (0.5 g / L) and Pd(II) (50 mg / L) in 100 mL of inorganic salt medium, the salinity is 2%, and the pH is 7.2, then add 2 g / L of sodium lactate, Nitrogen exposure for 10 min, at 30 o C in the dark incubator reaction for 36 h, the resulting mixture ( figure 1 A) Centrifuge (10000 rpm, 5 min), place the pellet in deionized water for 1 h, and centrifuge again to place the pellet at 60 o Drying in a C oven, the powder obtained through X-ray diffraction energy spectrum verification is the bionano palladium ( Figure 2A ).
[0044] Inorganic salt medium is per liter, 1 g NH4Cl, 0.8g Na2HPO4, 0.2g KH2PO4, 0.2g MgCl2 7H2O, 0.1 g CaCl2 2H2O, 20g NaCl, the balance is deionized water, the reaction temperature, salinity and pH are respectively for 30 o C, 2% and 7.2.
[0045] From the XRD standard collection of palladium (JCPDS 46-1043) recorded in the above figure, at ~40 o , 46 o , 68 o ,8...
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