A kind of marine bacteria and its application
A marine bacterium, a typical technology, applied in the direction of bacteria, special compound water treatment, microorganisms, etc., can solve the problems of no marine bacteria, etc., and achieve the effect of strong resistance
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Embodiment 1
[0032] Take 0.1g of sediment from the Bohai Strait (N 38°30.29', E 121°14.10') and inoculate it into LB medium (10g / L peptone, 5g / L yeast extract, 10g / L sodium chloride) on a shaker (150 rpM) cultured overnight at 37°C, and screened multiple strains of pure bacteria by the plate coating method. Further use the inorganic salt medium containing Au(III) as the test system to screen for the ability to reduce Au(III) to Au 0 (Appearance can change from colorless to pink or purple) strains for follow-up studies.
[0033] Based on the above background, the present invention uses the marine bottom mud in the middle of the Bohai Strait as the source of bacteria, and screens out a marine indigenous bacterium with Au(III) reducing ability, named CNZ-1, which is identified as Shewanella genus sp.), to be preserved in the China Center for Type Culture Collection, the address is Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province, the preservation time is 2017.10.08, a...
Embodiment 2
[0035] Mix the above-mentioned obtained strain CNZ-1 (0.5g / L) and Au(III) (50mg / L) in 100mL inorganic salt medium, the salinity is 2%, adjust the pH to 7, and then add 2g / L Different electron donors (fructose, lactose, potassium acetate, glucose, sodium formate, sodium lactate), exposed to nitrogen for 10 minutes, and reacted in the dark for 24 hours in an anaerobic incubator at 30 ° C, the color change see figure 1 .
[0036] The inorganic salt medium is per liter, 1g NH 4 Cl, 0.8gNa 2 HPO 4 ,0.2gKH 2 PO 4 , 0.2g MgCl 2 ·7H 2 O, 0.1g CaCl 2 2H 2 O, 20g NaCl, the balance is deionized water.
[0037] Depend on figure 1 It can be seen that CNZ-1 can reduce colorless Au(III) to pink or purple Au nanoparticles in the presence of various electron donors, and the darker the color, the better the reduction effect. The results show that sodium lactate and sodium formate are the best electron donors in the current reaction system.
Embodiment 3
[0039] Preparation of bionanogold:
[0040] Mix the above-mentioned obtained strain CNZ-1 (0.5g / L) and Au(III) (50mg / L) in 100mL inorganic salt medium, the salinity is 2%, adjust the pH to 7, and then add 2g / L sodium lactate, exposed to nitrogen for 10min, and reacted in the dark for 24h in a 30°C incubator, and the resulting mixture (see figure 1 ) centrifuge (10000rpm, 5min), place the precipitate in deionized water for ultrasonic treatment for 1h, centrifuge again and place the precipitate in a 60°C oven to dry, and the powder obtained through X-ray diffraction energy spectrum verification is the prepared bionano gold ( see figure 2 ).
[0041] The inorganic salt medium is per liter, 1g NH 4 Cl, 0.8gNa 2 HPO 4 ,0.2gKH 2 PO 4 , 0.2g MgCl 2 ·7H 2 O, 0.1g CaCl 2 2H 2 O, 20g NaCl, the balance is deionized water.
[0042] It can be known from the above figure that the 4f of Au 7 / 2The binding energy position appears at 83.7eV, while the 4f of Au(III) in the standard...
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