Method for rapidly propagating radix pseudostellariae seedlings

A technology of heterophylla and seedlings, applied in the field of plant tissue culture, can solve the problems of seed root quality degradation, mixed provenance, and yield decline, and achieve the effects of reducing plant viruses, healthy plant growth, and increasing yield

Inactive Publication Date: 2018-06-15
黔东南民族职业技术学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are two kinds of propagation methods of Pseudostellariae Pseudostellariae: seed propagation and tuber propagation. Because the fruit of Pseudostellariae Pseudostellariae is a capsule, the seeds ripen inconsistently, the fruit cracks, and the seeds will fall off naturally, making it difficult to harvest the seeds; in addition, the mature seeds of Pseudostellariae Pseudostellariae have dormancy characteristics , the seed germination rate is low, so roots are usually used in production for asexual reproduction (root propagation)
The most direct method of tuber root propagation is to use seed ginseng for propagation; however, in production, seed roots have been used for asexual reproduction for a long time, and viral diseases of heterophylla heterophylla are common, and the disease is becoming more and more serious. , the output has dropped sharply, and the enthusiasm of growers has been severely hit

Method used

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  • Method for rapidly propagating radix pseudostellariae seedlings
  • Method for rapidly propagating radix pseudostellariae seedlings
  • Method for rapidly propagating radix pseudostellariae seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Methods of explant treatment:

[0030] Take the vigorously growing winter buds of heterophylla as explants, cut them off from the base of 0.5-0.8cm, wash and soak for 6-10min, rinse with tap water for 30min, place on a super-clean workbench, soak in 75% alcohol for 30s, Rinse with sterile water 3-4 times, and then treat the buds of Pseudostellaria ginseng with different disinfection methods. The different disinfection methods are: ① soaking in 0.1% mercury chloride for 8 minutes; ② soaking in 0.1% mercury chloride for 10 minutes; ③ soaking in 0.1% mercury chloride for 12 minutes; Rinse 7-8 times with sterile water, dry the water with sterile paper, peel off the shoot tip of about 0.3-0.5mm in size, inoculate it on Pseudostellaria heterophylla adventitious bud induction medium, inoculate 20 bottles with each disinfection method, repeat 3 times, 20 The statistical results were analyzed in days later, and the results are shown in Table 1.

[0031] Table 1 Effect of disin...

Embodiment 2

[0035] The induction method of adventitious buds and the selection of medium thereof comprise the following steps:

[0036] 1) Explant treatment: Take the healthy winter buds of heterophylla heterophylla as explants, cut them off from the base of 0.5-0.8cm, wash and soak for 6-10min, rinse with tap water for 30min, place on the ultra-clean workbench, Soak in 75% alcohol for 30s, rinse with sterile water for 3-4 times, treat with 0.1% mercuric chloride for 12 minutes; then rinse with sterile water for 7-8 times, dry the water with sterile paper and peel off the stems of about 0.3-0.5 mm in size tip, inoculated on the adventitious bud induction medium of Pseudostellariae heterophylla.

[0037] 2) Induction of adventitious buds: inoculate the sterilized shoot tips into the various adventitious bud induction medium in table 2 based on MS, adding different concentrations of hormones and organic matter combinations, which also include sucrose 30g / L and Agar 4.0g / L, pH 5.6-5.8, inoc...

Embodiment 3

[0042] The method for inducing clustered buds and the selection of the culture medium thereof comprise the following steps:

[0043]1) Explant treatment: Take the healthy winter buds of heterophylla heterophylla as explants, cut them off from the base of 0.5-0.8cm, wash and soak for 6-10min, rinse with tap water for 30min, place on the ultra-clean workbench, Soak in 75% alcohol for 30s, rinse with sterile water for 3-4 times, treat with 0.1% mercuric chloride for 12 minutes; then rinse with sterile water for 7-8 times, dry the water with sterile paper and peel off the stems of about 0.3-0.5mm in size tip, inoculated on the adventitious bud induction medium of Pseudostellariae heterophylla.

[0044] 2) Induction of adventitious buds: Inoculate the sterilized shoot tips into MS+6-BA 2mg / L+NAA0.3mg / L+hydrolyzed casein 0.3g / L+sucrose 30g / L+agar 4.0g / L, pH5.8 In the culture medium, the culture condition temperature is 25±1°C, the light time is 12h.d-1, and the light intensity is 1...

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Abstract

The invention relates to a method for rapidly propagating radix pseudostellariae seedlings. The method comprises the following steps: performing sterile treatment on winter buds of radix pseudostellariae as explants, performing tissue culture such as adventitious bud induction, performing multiple shoot induction and adventitious root induction, and further performing domestication and transplanting. By optimizing specific parameters of different steps and combination of culture medium components, the quality of radix pseudostellariae test tube seedlings can be improved, the survival rate of the radix pseudostellariae test tube seedlings can be further increased after transplanting, plants can grow healthy, underground earthnuts can be enlarged, and the yield of the radix pseudostellariaecan be increased. A reliable method is provided for industrial production of radix pseudostellariae, and long-term stable development of the radix pseudostellariae industry can be promoted.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for rapid propagation of heterophylla heterophylla seedlings. Background technique [0002] Pseudostellaria heterophylla is a commonly used traditional Chinese medicine, also known as baby ginseng, child ginseng, double batch seven, four-leaf ginseng or rice ginseng. root. Its root tuber is used as medicine. It is a precious Chinese medicinal material. It has mild medicinal properties and can be used as a substitute for ginseng and even American ginseng. It is suitable for people of all ages. The market demand is high. Guizhou Province is one of the main production areas of medicinal materials in my country, and Shibing County of Guizhou Province has become one of the main production areas of heterophylla heterophylla in the country. At present, there are two kinds of propagation methods of Pseudostellariae Pseudostellariae: seed propagation and tuber propa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄刚郑晓峰付燕周黎万新屏何宪江
Owner 黔东南民族职业技术学院
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