Rapid propagation method for tissue culture of golden foster holly seedlings

Abstract

The invention provides a rapid propagation method for tissue culture of golden foster holly seedlings. New shoots of healthy plants of biennial or triennial golden foster holly are adopted as explants, an adventitious bud induction pathway is adopted, and the purpose of rapid propagation is achieved by using adventitious buds with the length of a stem segment. The method comprises the following steps of selection and disinfection of the explants, induction culture of the adventitious buds, proliferation culture of stem segments and shoot tips, rooting culture and transplanting of test tube seedlings. It takes only 150-180 days to obtain the golden foster holly seedlings by means of the method; in the propagation process, not only are the explants low in pollution rate and high in inductionrate and proliferation rate, but also the subsequent rooting culture can be carried out without strong seedling culture after propagation, so that the cultivation process is simplified, the cultivation cost is reduced, and correspondingly the efficiency of culturing the whole seedlings is improved. In addition, the test tube seedlings cultured by the method are robust, roots are robust, the environment adaptability is high through domestication and hardening of the seedlings, and therefore the survival rate of the seedlings after transplanting is increased.

Description

【Technical field】 [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method for tissue culture of golden wolfberry seedlings. 【Background technique】 [0002] Ilex attenuata 'Sunny Foster', also known as narrow-leaf holly and narrow-crown holly, is a natural hybrid of Hongda holly (Ilex cassine) and American holly (Ilex opaca). Golden wolfberry is the latest small tree species introduced from abroad. It has the characteristics of no thorns, strong branching, golden leaf color, and red fruit color. It can be used as a color block for roads, parks, courtyards, short fences, isolated plant greening, etc. The domestic seedling market demand is increasing, and the demand for high-quality seedlings is urgent. [0003] At present, the production of golden wolfberry seedlings mainly depends on seed sowing and cutting propagation. The emergence rate of seed sowing is low. Cutting propagation not only requires ...

AI Technical Summary

Problems solved by technology

[0005] The Chinese wolfberry seedling raising technology that provides in the above literature uses seeds as explants, but there are following defects usually: the seedling consistency of propagation can't be guaranteed, need to induce aseptic seedling through seed simultaneously, induce bunchy bud, through bunchy bud Proliferation, rooting and other processes, the seedling raising process is cumbersome; the explant contamination rate of the thornless Chinese wolfberry and the Chinese Chinese wolfberry rapid propagation technology is high, and after the test tube seedlings take root in the bottle, cuttings need to be rooted outside the bottle, the seedling raising process is not only cumbersome , and the rooting rate is low, and the reproductive efficiency is low;