Compound immune magnetic beads for separation and purification of hepatic stellate cells and preparation method of compound immune magnetic beads
A technology of hepatic stellate cells and immunomagnetic beads, which is applied in the field of composite immunomagnetic beads and their preparation, can solve the problems of poor antibody stability, low antibody grafting rate, easy agglomeration, etc., achieve good suspension and dispersion, and improve dispersion performance. , the effect of reducing weight
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[0028] Example one
[0029] Fe 3 O 4 Preparation of microspheres: take 0.1moL Fe 2 (SO 4 ) 3 And 0.1moL FeSO 4 ·7H 2 O mix well, add 500mL of distilled water and stir until it is completely dissolved to obtain a mixed solution, take 100g of egg white liquid and stir it into a milky white uniform state, stir and add it dropwise to the mixed solution, stir and react for 30h at 60-65℃, and dry under reduced pressure , Grind, and transfer to 450 ℃ muffle furnace for 6 hours, then take out and cool down with a mixture of ice and water, wash twice with distilled water and absolute ethanol, dry the powder into the reactor, and put it in argon Under the conditions of flow rate 80sccm, pressure 3Pa, power 80W, plasma treatment for 10min to obtain Fe 3 O 4 Microspheres.
[0030] The preparation of carboxylated graphene is as follows: Take graphene and add it to 0.3 mol / L sodium hydroxide solution to ultrasonically disperse for 3 hours, then add chloroacetic acid to ultrasonically react for 3...
Example Embodiment
[0035] Example two
[0036] Fe 3 O 4 Preparation of microspheres: take 0.1moL Fe 2 (SO 4 ) 3 And 0.1moL FeSO 4 ·7H 2 O mix well, add 500mL of distilled water and stir until it is completely dissolved to obtain a mixed solution, take 100g of egg white liquid and stir it into a milky white uniform state, stir and add it dropwise to the mixed solution, stir and react for 30h at 60-65℃, and dry under reduced pressure , Grind, and transfer to 450 ℃ muffle furnace for 6 hours, then take out and cool down with a mixture of ice and water, wash twice with distilled water and absolute ethanol, dry the powder into the reactor, and put it in argon Under the conditions of a flow rate of 120sccm, a pressure of 8Pa, and a power of 100W, plasma treatment is performed for 15 minutes to obtain Fe 3 O 4 Microspheres.
[0037] The preparation of carboxylated graphene is the same as in Example 1.
[0038] Graphene / Fe 3 O 4 Preparation of microspheres: take 1.5g Fe 3 O 4 The microspheres and 2.0 g carbox...
Example Embodiment
[0042] Example three
[0043] Fe 3 O 4 Preparation of microspheres: take 0.1moL Fe 2 (SO 4 ) 3 And 0.1moL FeSO 4 ·7H 2 O mix well, add 500mL of distilled water and stir until it is completely dissolved to obtain a mixed solution, take 100g of egg white liquid and stir it into a milky white uniform state, stir and add it dropwise to the mixed solution, stir and react for 30h at 60-65℃, and dry under reduced pressure , Grind, and transfer to 450 ℃ muffle furnace for 6 hours, then take out and cool down with a mixture of ice and water, wash twice with distilled water and absolute ethanol, dry the powder into the reactor, and put it in argon Under the conditions of a flow rate of 100 sccm, a pressure of 5 Pa, and a power of 90 W, plasma treatment was performed for 13 minutes to obtain Fe 3 O 4 Microspheres.
[0044] The preparation of carboxylated graphene is the same as in Example 1.
[0045] Graphene / Fe 3 O 4 Preparation of microspheres: take 2.0g Fe 3 O 4 Microspheres and 3.5 g carbo...
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