Methods for predicting palm oil yield of a test oil palm plant

A technology for experimental oil and palm oil, which can be used in biochemical equipment and methods, microbial determination/inspection, bioinformatics, etc., and can solve problems such as narrow breeding materials

Inactive Publication Date: 2018-07-17
SIME DARBY PLANTATION INTPROP SDN BHD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, applying this approach to oil palms is impractical because commercial palms tend to arise from genetically narrow breeding material

Method used

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  • Methods for predicting palm oil yield of a test oil palm plant
  • Methods for predicting palm oil yield of a test oil palm plant
  • Methods for predicting palm oil yield of a test oil palm plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0207] Sampling and DNA preparation

[0208] Genome-wide association study (also known as GWAS) mapping populations derived from the Ulu Remis dura x AVROS pisifera population (1,218 palms) and the Banting durax AVROS pisifera population (953 palms) were sampled. Sample selection was based on good representatives of the oil / plant palm plant (also known as O / P) variant and pedigrees recorded by the corresponding breeders. Total genomic DNA was isolated from unexpanded young leaves using the DNAeasy(R) Plant Mini Kit (Qiagen, Limburg, The Netherlands).

[0209] whole genome resequencing

[0210] The samples were pooled based on equal molar concentrations of DNA from each sample to form a sequencing DNA pool. Resequenced libraries were prepared using the HiSeq2000(TM) Sequencing System (Illumina, San Diego, CA) to generate 100 bp paired-end reads to 35x genome coverage, resulting in 1,015,758,056 raw reads. Using BWAMapper (published as Li & Durbin, Bioinformatics 26: 589-595 ...

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Abstract

Methods for predicting palm oil yield of a test oil palm plant are disclosed. The methods comprise determining, from a sample of a test oil palm plant of a population, at least a first SNP genotype, corresponding to a first SNP marker, located in a first QTL for a high-oil- production trait and associated, after stratification and kinship correction, with the high-oil- production trait with a genome-wide -log10(p-value) of at least 3.0 in the population or having a linkage disequilibrium r2 value of at least 0.2 with respect to a first other SNP marker linked thereto and associated, after stratification and kinship correction, with the high-oil-production trait with a genome-wide -log10(p-value) of at least 3.0 in the population. The methods also comprise comparing the first SNP genotype to a corresponding first reference SNP genotype and predicting palm oil yield of the test plant based on extent of matching of the SNP genotypes.

Description

technical field [0001] The present application relates to a method for predicting palm oil yield of experimental oil palm plants, and more particularly, to a method for predicting palm oil yield of experimental oil palm plants comprising experimental oil palm from a population of oil palm plants Determine at least the first single nucleotide polymorphism (SNP) genotype of the experimental oil palm plant in the sample of the plant, and the first SNP genotype corresponds to the first SNP marker; the first SNP gene of the experimental oil palm plant The genotype is compared with a corresponding first reference SNP genotype indicative of a high oil yield trait in the same genetic background as the population; and based on the degree of matching of the first SNP genotype of the experimental oil palm plant to the corresponding first reference SNP genotype to predict the palm oil yield of the experimental oil palm plant. Background technique [0002] African oil palm Elaeis guinee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895G16B20/00
CPCC12Q2600/13C12Q2600/156G16B20/00C12Q1/6895
Inventor Q·B·克翁A·L·龙C·K·特M·穆罕默德F·T·周D·R·阿普尔顿H·库拉维拉辛加姆
Owner SIME DARBY PLANTATION INTPROP SDN BHD
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