Preparation method of thamnaconus multiradiatus fish skin bacterium-inhibiting peptide

Abstract

The invention provides a preparation method of thamnaconus multiradiatus fish skin bacterium-inhibiting peptide. The preparation method comprises the following steps: a) pre-treating fish skin: mincing the thamnaconus multiradiatus fish skin; then carrying out degreasing and calcium removal in sequence to obtain degreased and calcium-removed fish skin; then removing non-collagen to obtain degreased, calcium-removed and non-collagen-removed thamnaconus multiradiatus fish skin; b) carrying out enzymolysis: adding the degreased, calcium-removed and non-collagen-removed thamnaconus multiradiatus fish skin which is sufficiently dried into a buffering solution according to the material / liquid ratio of 1g to (8 to 12mL); regulating the pH (Potential of Hydrogen) value to 6.5 to 7.5; carrying outheat preservation at 45 to 55 DEG C for 10 to 15min; adding compound proteinase according to 2 to 5 percent of the mass of the fish skin and carrying out the enzymolysis at 45 to 55 DEG C for 4 to 6h;c) preparing the bacterium-inhibiting peptide: deactivating an enzymolysis solution in a water bath for 10 to 15min and obtaining supernatant through ultrasonic extraction or microwave extraction; carrying out ultra-filtration and chromatography or ultra-filtration, spraying and drying on the supernatant in sequence to obtain the thamnaconus multiradiatus fish skin bacterium-inhibiting peptide. The preparation method provided by the invention has the characteristics that a preparation technology is simple, an enzymolysis or hydrolysis method is used for specifically obtaining hydrolyzed polypeptide with a bacterium-inhibiting effect and the effect of the proteinase on fish skin protein is promoted so that efficient preparation of the bacterium-inhibiting peptide is realized and the like.

Description

technical field

[0001] The invention relates to a biologically active peptide and its preparation method and use, in particular to a horse-faced puffer fish skin antioxidant collagen peptide and its preparation method and use. Background technique

[0002] Horse-faced puffer is a common fish in my country. The skin of horse-faced puffer is thick and hard, so after fishing, if time permits, the skin will be peeled on the spot, and its meat will be refrigerated in storage until it is eaten, and its skin will be thrown away on the spot, resulting in waste of resources and environmental pollution. At present, there have been researches on the use of horse-faced fish skin for the leather industry, the preparation of collagen, and the separation and purification of arginine and proline. Using horse-faced puffer fish skin as raw material and using enzymatic hydrolysis technology to develop collagen peptides will also lay a foundation for the high-value utilization of horse-faced p...

Examples

Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific embodiment: a kind of preparation method of horse-faced fish skin bacteriostatic peptide, comprises the following steps:

[0016] a) Fish skin pretreatment: mince the fish skin of horse-faced puffer fish, then degrease and decalcify in sequence to obtain degreased and decalcified fish skin; puffer fish skin;

[0017] b) Enzymatic hydrolysis: add the fully dried degreased, decalcified and decollagenized horse-faced puffer fish skin into the buffer solution according to the ratio of material to liquid 1g:8-12mL, adjust the pH value to 6.5-7.5, and put it at 45-55 Keep warm at ℃ for 10-15 minutes, add compound protease according to 2-5% of the mass of fish skin, and enzymolyze at 45-55℃ for 4-6 hours;

[0018] c) Preparation of bacteriostatic peptide: inactivate the enzymatic hydrolysis solution in a boiling water bath for 10-15 minutes, then extract the supernatant by ultrasonic extracti...

technical field

[0001] The invention relates to a biologically active peptide and its preparation method and use, in particular to a horse-faced puffer fish skin antioxidant collagen peptide and its preparation method and use. Background technique

[0002] Horse-faced puffer is a common fish in my country. The skin of horse-faced puffer is thick and hard, so after fishing, if time permits, the skin will be peeled on the spot, and its meat will be refrigerated in storage until it is eaten, and its skin will be thrown away on the spot, resulting in waste of resources and environmental pollution. At present, there have been researches on the use of horse-faced fish skin for the leather industry, the preparation of collagen, and the separation and purification of arginine and proline. Using horse-faced puffer fish skin as raw material and using enzymatic hydrolysis technology to develop collagen peptides will also lay a foundation for the high-value utilization of horse-faced p...

Examples

Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with specific embodiment: a kind of preparation method of horse-faced fish skin bacteriostatic peptide, comprises the following steps:

[0016] a) Fish skin pretreatment: mince the fish skin of horse-faced puffer fish, then degrease and decalcify in sequence to obtain degreased and decalcified fish skin; puffer fish skin;

[0017] b) Enzymatic hydrolysis: add the fully dried degreased, decalcified and decollagenized horse-faced puffer fish skin into the buffer solution according to the ratio of material to liquid 1g:8-12mL, adjust the pH value to 6.5-7.5, and put it at 45-55 Keep warm at ℃ for 10-15 minutes, add compound protease according to 2-5% of the mass of fish skin, and enzymolyze at 45-55℃ for 4-6 hours;

[0018] c) Preparation of bacteriostatic peptide: inactivate the enzymatic hydrolysis solution in a boiling water bath for 10-15 minutes, then extract the supernatant by ultrasonic extracti...