SNP molecular marker related to sheep kidding number properties, and applications thereof

A technology of molecular markers and litter size, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of difficult high-throughput automatic measurement, low throughput, and numerous procedures, and achieve Improve fecundity, high accuracy, cost-effective effect
CN108315435AActive Publication Date: 2018-07-24INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2018-07-24

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Abstract

The invention provides a SNP molecular marker related to sheep kidding number properties, and applications thereof. The SNP molecular marker related to sheep kidding number properties is the 4389th base, which is A or G, of GLIS1 gene sequence represented by SEQ ID NO:1 of sheep. The invention also discloses a method used for detecting sheep GLIS1 gene type and predicting sheep average kidding number based on Sequenom MassARRAY register SNP technology. According to the method, the nucleotide at the 27726261bp site of the No.1 chromosome of sheep is subjected to mononucleotide type detection, and it is determined whether the sheep GLIS1 gene is a homozygous gene or a heterozygous gene based on the mononucleotide type detection results. GC type homozygous individuals with high kidding numberproperties and AA type homozygous individuals with high kidding number are selected and reserved through selection on sheep GLIS1 gene, GA heterozygous individuals with low kidding number is weeded out, so that sheep breeding capacity is improved, and potential application value on sheep large scale molecular breeding is achieved.
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Description

technical field

[0001] The invention relates to a molecular marker detection technology, in particular to a SNP molecular marker related to the litter size trait of sheep and its application. Background technique

[0002] The litter size trait is one of the most important economic traits of sheep. Because it is controlled by micro-effect polygenes and has low heritability, it is difficult to improve rapidly by conventional breeding methods. Molecular technology can effectively and rapidly improve its genetic progress, so the identification Major genes or molecular genetic markers related to litter size are the key to modern molecular breeding.

[0003] GLIS1 is the GLIS Family Zinc Finger 1 (GLIS Family Zinc Finger 1, GLIS1) gene, which is a protein highly expressed in the first and second cell stages of mouse oocytes and fertilized eggs, and plays an important role in mouse embryonic development. effect. The GLIS1 gene plays an important role in the reprogramming process ...

Claims

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