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Use of klotho protein in preparation of reagents for diagnosing risk of gastrointestinal stromal tumors

A technology of gastrointestinal stromal tumors and risk, which is applied in the fields of medicine and clinical diagnosis, and can solve the problems of research reports on the correlation between klotho and gastrointestinal stromal tumors, etc.

Active Publication Date: 2020-05-26
北京科跃中楷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Searching the domestic and foreign literature and patents of the prior art, there is no research report on the correlation between klotho and gastrointestinal stromal tumors, and there is no KLOTHO protein and its related components used in gastrointestinal stromal tumors Research reports on tumor diagnosis and treatment

Method used

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  • Use of klotho protein in preparation of reagents for diagnosing risk of gastrointestinal stromal tumors
  • Use of klotho protein in preparation of reagents for diagnosing risk of gastrointestinal stromal tumors
  • Use of klotho protein in preparation of reagents for diagnosing risk of gastrointestinal stromal tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Clinical Significance of KLOTHO Protein Expression in GISTs Tissues

[0019] (1) Clinicopathological samples

[0020] The GISTs sample used for detection is a tissue chip composed of 90 cases of GISTs tissue, which was purchased from Shanghai Xinchao Biological Co., Ltd., item number HDgS-GIST090Loc-01, with relevant clinical and pathological data.

[0021] (2) Experimental method

[0022] Immunohistochemical S-P method was used to detect the expression of KLOTHO protein in tissue chips composed of 90 cases of GISTs. Proceed as follows:

[0023] 1. Put the tissue chip into the oven, adjust the temperature to 63 degrees, and bake the wax for one hour.

[0024] 2. Preparation of reagents:

[0025] 2.1 10*PBS buffer (recipe: 80g NaCl, 2g KCl, 15.35g Na2HPO4, 2g KH2PO4, dilute to 1000 ml with pure water): dilute 10*PBS buffer to 1*PBS buffer, and then Add Tween reagent accounting for 0.05% of the total volume to the PBS buffer;

[0026] 2.2 Antigen retrieval...

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Abstract

The invention relates to a use of KLOTHO protein to estimation of the risk degree of gastrointestinal stromal tumor, and concretely provides a use of KLOTHO protein to preparation of a reagent for diagnosing the risk degree of gastrointestinal stromal tumor. The amino acid sequence of the KLOTHO protein is shown as SEQ NO.1. The use has the following technical effects that when the risk degree ofthe gastrointestinal stromal tumor of a patient with the gastrointestinal stromal tumor is higher, the KLOTHO protein expression positive rate in the gastrointestinal stromal tumor tissue is higher; the risk degree of the gastrointestinal stromal tumor is predicted through detecting the KLOTHO protein in the individual gastrointestinal stromal tumor tissues and the relevant composition ingredients.

Description

technical field [0001] The invention relates to the fields of medicine and clinical diagnosis. Specifically, it relates to the use of a KLOTHO protein as a marker in diagnosing the risk of gastrointestinal stromal tumors. Background technique [0002] Gastrointestinal stromal tumor (GIST) is a type of mesenchymal tumor originating from the gastrointestinal tract. Different from malignant tumors and cancers in the traditional sense, its biological behavior and clinical manifestations are complex and diverse. Covering benign, low-grade malignancy, moderate malignancy, and high-grade malignancy, the prognosis of patients with GISTs is also very different. Benign GISTs can live without any progression, while high-grade GISTs grow very fast and have a very poor prognosis. For the judgment of the risk of GISTs, the National Institutes of Health (NIH) risk classification is generally used at present, that is, the judgment is mainly based on tumor size, mitotic phase, and primary t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446G01N33/57484G01N2333/575
Inventor 黄曙林洁王敏朱苏敏
Owner 北京科跃中楷生物技术有限公司