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Anti-IL-4R antibody and application thereof

An IL4, antibody technology, applied in the direction of antibody, application, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, etc.

Active Publication Date: 2018-08-07
BEIJING WISDOMAB BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These studies suggest that blocking IL-4R may be effective in the treatment of AD

Method used

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  • Anti-IL-4R antibody and application thereof
  • Anti-IL-4R antibody and application thereof
  • Anti-IL-4R antibody and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Example 1. Construction of Phage Display Antibody Library

[0138] The content of this example refers to the inventor’s previous Chinese patent applications No. 201610609651.X (titled: Anti-human PDL1 antibody and its use) and No. 201510097117.0 (titled: Anti-human IL-17 monoclonal antibody). By way of reference, the contents of the above two patent applications are incorporated herein.

Embodiment 2

[0139] Embodiment 2: Preparation of recombinant protein

[0140] A variety of different recombinant proteins were used in the preparation and testing of anti-IL-4R mAbs, including human IL-4R extracellular domain (hIL-4R, SEQ ID NO:1), mouse IL-4R extracellular domain ( mIL-4R, SEQ ID NO:2), macaque IL-4R extracellular region (mmIL-4R, SEQ ID NO:3), human IL-4 extracellular region (hIL-4, SEQ ID NO:4) and human Recombinant IL-13 (SEQ ID NO:32). These proteins all have post-translational modifications (such as: glycosylation or disulfide bonds, etc.), so the use of mammalian cell expression systems will be more conducive to maintaining the structure and function of recombinant proteins. In addition, a His tag (His, SEQ ID NO: 5) or the Fc fragment of human antibody IgG1 (Fc, SEQ ID NO: 6) or the Fc fragment of mouse IgG2a (mFc, SEQ ID NO: 6) was added to the C-terminus of these recombinant proteins. NO:7), which is more conducive to the purification of recombinant proteins an...

Embodiment 3

[0143] Example 3: Screening of anti-human IL4R monoclonal antibody using phage display antibody library technology

[0144] 3.1 Screening of anti-human IL4R monoclonal antibody

[0145] Using the recombinant hIL4R-his prepared in Example 2 as an antigen, a solid-phase screening strategy was used (for the experimental scheme, refer to Phage Display: General Experimental Guidelines / (US) Clarkson (Clackson, T.), (US) Loman (Lowman, H.B. ) edited; translated by Ma Lan et al. Chemical Industry Press, 2008.5) screened the phage library displaying the human single-chain antibody library prepared in Example 1, and obtained 3 human antibodies with different sequences but all capable of specifically binding to human IL4R, including Clone S1E6 (amino acid sequence is SEQ ID NO: 17, VH sequence is SEQ ID NO: 18, VL sequence is SEQ ID NO: 19), S1H9 (amino acid sequence is SEQ ID NO: 20, VH sequence is SEQ ID NO: 21 , VL sequence is SEQ ID NO:22), S2C2 (amino acid sequence is SEQ ID NO:23,...

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PUM

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Abstract

The invention discloses an antibody combining human IL-4R or an antigen binding part thereof, polynucleotide coding the antibody or the antigen binding part thereof, a carrier containing the polynucleotide, host cells containing the polynucleotide or carrier, a method for preparing and purifying the antibody and application of the antibody or the antigen binding part thereof.

Description

technical field [0001] The present application generally relates to the field of genetic engineering and antibody medicine; specifically, it relates to the field of anti-human interleukin-4 receptor (IL-4R) antibody and its application. The present application develops a new anti-human IL-4R antibody, and provides the use of the antibody in the treatment of IL-4R-mediated diseases. Background of the invention [0002] Interleukin-4 (IL-4) consists of 153 amino acids with a molecular weight of about 17 kDa. Initially, IL-4 was discovered because of its ability to stimulate B cell proliferation and was named B cell stimulating factor-1 (BSF-1) [1] . IL-4, like IL-13, belongs to the type I cytokine family, and has a quaternary structure composed of a hydrophobic bundle core of 4α helices [2] . IL-4 is secreted by TH2 cells, participates in TH2-mediated immune response, and has a wide range of biological activities, including stimulating the proliferation of T cells, mast ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C12N15/13A61K39/395A61P37/02A61P11/06A61P37/08A61P17/00
CPCA61P11/06A61P17/00A61P37/02A61P37/08C07K16/2866A61K2039/505C07K2317/56C07K2317/76C07K2317/565C07K2317/92C07K2317/622C07K2317/21
Inventor 刘志刚刘玉兰郝小勃蒋磊郭晶晶
Owner BEIJING WISDOMAB BIOTECHNOLOGY CO LTD
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