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Triazole-degrading bacteria and its application in the treatment of triazole-containing wastewater

A technology for wastewater treatment and triazole, which is used in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc., and can solve problems such as refractory degradation and triazole toxicity.

Active Publication Date: 2020-07-31
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the toxicity and refractory characteristics of triazoles, it is necessary to find strains that can tolerate the biotoxicity of triazoles and have degradation functions

Method used

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  • Triazole-degrading bacteria and its application in the treatment of triazole-containing wastewater
  • Triazole-degrading bacteria and its application in the treatment of triazole-containing wastewater
  • Triazole-degrading bacteria and its application in the treatment of triazole-containing wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Screening, isolation and identification of Raoultella sp.NJUST42.

[0019] (1) Isolation of strains

[0020] Take 10mL of activated sludge from the existing activated sludge reactor used to treat triazole wastewater in the laboratory, add it to 100mL of normal saline, stir evenly, and let it stand for two hours. Take 1 mL of the supernatant and add it to 50 mL of inorganic salt medium, enrich and cultivate in a constant temperature shaking incubator for three days (180 rpm), after three consecutive enrichments, take the culture solution and dilute it to 10 -4 -10 -10 times. Prepare inorganic salt agar solid medium plates, spread 20 μL of the diluted culture solution on the inorganic salt agar solid medium, and place them in a biochemical incubator at 30°C for three days. Select the single colonies with obvious differences on the petri dish, and use the method of plate streak separation to carry out purification and culture. After five consecutive purifications, a sin...

Embodiment 2

[0031] Degradation performance of strain Raoultella sp.NJUST42 on triazoles.

[0032] Inoculate strain Raoultella sp.NJUST42 to contain 50mg L -1In the LB medium of triazole, culture on a shaker at 180 rpm at 35°C to enrich the NJUST42 strain, and after the strain enters the logarithmic growth phase (about 48 hours), centrifuge the obtained bacterial solution for 10 Minutes (6000 rev / min), obtain deposition thalli, resuspend with the inorganic salt liquid culture medium after the sterilization, centrifugal, wash repeatedly three times, the thalline resuspends in the aseptic liquid inorganic salt medium, obtains seed liquid ( Control OD 600 about 1.5).

[0033] Prepared with 50mg L -1 Inorganic salt liquid medium in which triazole is the only carbon source and nitrogen source is used as simulated wastewater, the above seed liquid is added to simulated triazole wastewater, the inoculum size is 5%, and the rotation speed is 180 rpm under the condition of 35°C Cultivate on a s...

Embodiment 3

[0035] Example 3 Effect of temperature and initial pH value on triazole degradation performance.

[0036] Prepare the triazole concentration as 50mg L -1 , triazole simulated wastewater with an initial pH value of 7.0, Raoultellasp. cultured in a shaker. Depend on Figure 4 It was shown that Raoultellasp.NJUST42 can efficiently remove triazole under the condition of culture temperature of 30℃~35℃, and the removal effect of triazole is the best under the condition of culture temperature of 35℃; Under the conditions of 20℃, 25℃ and 40℃, Raoultella sp.NJUST42 can also remove triazole, but the removal rate is significantly slower.

[0037] Prepare the triazole concentration as 50mg L -1 , triazole simulated wastewater with initial pH of 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0, Raoultella sp. Depend on Figure 5 As shown, under the condition of initial pH of 7.0 and 8.0, Raoultella sp.NJUST42 can realize the efficient removal of triazole, and the removal effect of triazole is the be...

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Abstract

The invention discloses a triazole-degrading bacterium and its application in the treatment of triazole-containing wastewater. In the present invention, the activated sludge in the activated sludge reactor for removing triazole is used as the screening bacteria source, and the screening medium using triazole as the only carbon source and nitrogen source is used for enrichment, and the medium is used for marking According to the method of separation and purification, a triazole-degrading strain was obtained, which was identified as Raoultella by molecular biology, named Raoultella sp. NJUST42, and the preservation number was CCTCC NO: M 2018050. The triazole-degrading bacteria of the present invention can use triazole as the sole carbon source and nitrogen source for metabolism and growth. When Raoultellasp.NJUST42 is inoculated into the actual industrial wastewater containing triazole, the removal rate of triazole can reach more than 50% within 108 hours, and it has a good application prospect in the wastewater treatment of triazole.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a triazole-degrading bacterium and its application in the treatment of triazole-containing wastewater. Background technique [0002] Triazole, as an important nitrogen-heterocyclic compound with broad-spectrum biological activity, can produce and synthesize a variety of compounds, including insecticides, herbicides, fungicides, plant growth regulators, and anti-tumor, anti-virus and Antibacterial agents, etc., are widely used in pharmaceutical, pesticide, chemical and other industries. More than 200,000 triazole derivatives have been reported. Triazole and its derivatives have stable structures, are not easily hydrolyzed, are easily transferred between soil and groundwater, and have biological toxicity, carcinogenicity and teratogenic properties. Therefore, remediation of the soil and water environment polluted by tria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 孙鹏翔孙倩倩王静沈锦优林晓璐罗哲江心白侯成吴浩博
Owner NANJING UNIV OF SCI & TECH
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