A kit for studying ubiquitin-like system in bacteria and its application

A kit and ubiquitin-like technology, applied in the field of biology, can solve problems such as unclear reasons, poor expression and solubility
CN108414772BActive Publication Date: 2021-02-09HENAN UNIV OF SCI & TECH
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN UNIV OF SCI & TECH
Publication Date
2021-02-09

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a kit for studying the ubiquitin-like system in bacteria and its application, belonging to the technical field of biology. The test kit of the present invention includes ThiS protein and ThiF protein, and can also include MgCl 2 and Tris‑HCl reaction buffer, DTT and ATP. ThiF protein has the function of ligase. In vitro, in the presence of ATP, ThiS protein can be connected to substrate protein to form ubiquitin-like modification, and this ubiquitin-like modification has substrate selectivity. FtsA and PriC can Modified by ThiS and ThiF proteins, but MoaC cannot. The kit of the invention can effectively study the ubiquitin-like modification of the substrate, and is convenient, quick and efficient.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a kit for studying the ubiquitin-like system in bacteria and its application, belonging to the technical field of biology. Background technique

[0002] Escherichia coli ubiquitin-like protein ThiS is a small molecule protein with a molecular weight of 7.2kDa. Its structure is very similar to that of ubiquitin protein. They all have five β sheets and two α helices. Previous studies have found that it is involved in the synthesis of vitamin B1 ( Iyer, L.M., Burroughs, A.M. & Aravind, L. The prokaryotic antecedents of the ubiquitin-signaling system and the early evolution of ubiquitin-like β-grasp domains. Genome Biology 7, 91-96 (2006)). ThiS and ThiF proteins are mainly involved in the synthesis of vitamin B1 in vivo. ThiF can activate the two GGs at the end of ThiSC, and then participate in the sulfur transport process. The main function of ThiF protein is to adenylylate ThiS protein (activation function) when vitamin B1 is sy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More