Sex pheromone for controlling phauda flammans walker and preparation method of sex pheromone lure
A technology of vermilion moth and pheromone, which is applied in the field of insect sex pheromone preparation, can solve the problems of vermilion moth producing drug resistance, threatening human health, killing natural enemies by mistake, etc., and achieves environmental friendliness, strong stress resistance, Long lasting effect
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Embodiment 1
[0016] Extraction of sex pheromone from the vermilion moth
[0017] Using headspace adsorption method and gonad extraction method to extract sex pheromones from unmated females of Trichoma vermilion with different ages and different times of entering the photoperiod, and detect them by gas chromatography-mass spectrometry and insect antennae potential measurement The system test shows that the sex information of the vermilion moth is composed of cis-9-hexadecenal and (Z,Z,Z)-9,12,15-octadecatrienal.
Embodiment 2
[0019] Preparation of Sex Pheromone Lures for Trichomonas vermilion
[0020] At room temperature, first mix 10 μl of cis-9-hexadecenal and 10 μl of (Z,Z,Z)-9,12,15-octadecatrienal into 980 μl of In the n-hexane solution, a mixed solution of two sex pheromones with a mass ratio of 1:1 is formed; secondly, the mixed solution containing the sex pheromone of Vermilion Trichodidae is added dropwise on the cotton sliver lure, until the mixed solution containing Vermilion Trichodia moth After the n-hexane in the sex pheromone mixture volatilizes, the lure containing the vermilion trichophyll moth attractant is prepared; finally, the prepared lure is put into an aluminum foil and sealed, and refrigerated at -20°C.
Embodiment 3
[0022] Trapping effect of sex pheromone in the vermilion moth in the wild
[0023] Set the following 8 lures:
[0024] A. Add 10 microliters of cis-9-hexadecenal to 990 microliters of n-hexane solution, add the mixed solution dropwise to the cotton sliver lure, and after the n-hexane volatilizes, the vermilion hair containing lures for moth attractants;
[0025] B. Add 10 microliters of (Z,Z,Z)-9,12,15-octadecatrienal to 990 microliters of n-hexane solution, and add the mixture dropwise to the cotton sliver lure, wait until After the normal hexane volatilizes, the lure core containing the vermilion trichod moth attractant is produced;
[0026] C. Add 10 microliters of trans-9-hexadecenal to 990 microliters of n-hexane solution, add the mixed solution dropwise to the cotton sliver lure, and after the n-hexane volatilizes, the vermilion hair lures for moth attractants;
[0027] D, add 10 microliters of cis-9-hexadecenal and 10 microliters of (Z, Z, Z)-9,12,15-octadecatrienal...
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