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A kind of compound bacterial agent with both functions of dissolving phosphorus and degrading dehp and its application

A compound bacterial agent and phosphorus-dissolving technology, applied in the field of microorganisms, can solve the problems of unstable effect, single performance, weak adaptability, etc., and achieve the effect of simple and easy cultivation method, good synergy and strong adaptability.

Active Publication Date: 2020-05-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the single performance of a single microbial agent, weak adaptability, unstable effect, the complexity of the soil, water and other environments where the microbial agent is applied, and the increasingly urgent demand for multifunctional agents, it is urgent to use the agent Combining, cultivating and producing a multifunctional compound bacterial agent with stable function, strong adaptability and low price

Method used

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  • A kind of compound bacterial agent with both functions of dissolving phosphorus and degrading dehp and its application
  • A kind of compound bacterial agent with both functions of dissolving phosphorus and degrading dehp and its application
  • A kind of compound bacterial agent with both functions of dissolving phosphorus and degrading dehp and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The Pseudomonas prosekii YLYP6 and Rhodococcus pyridinivorans XB antagonistic experiments:

[0037] Pseudomonas prosekii YLYP6 and Rhodococcuspyridinivorans XB were cross-streaked on LB solid medium by plate streaking method, and cultured at 30°C for 3 days. The results showed that the growth status of the two bacteria did not affect each other ( figure 1 ). Scanning electron microscopy was used to observe that the two strains could coexist in LB liquid medium ( figure 2 ), suitable for compatibility to form a composite microbial agent.

Embodiment 2

[0039] Phosphorus-dissolving ability analysis of compound bacterial agent:

[0040] (1) Inoculate Pseudomonas prosekii YLYP6 and Rhodococcus pyridinivorans XB in LB liquid medium respectively, and activate at 28°C for 12 hours;

[0041] (2) Pick two activated single bacteria and inoculate them in LB liquid medium respectively, and culture them until the number of bacteria is 10 8 cfu / mL;

[0042] (3) Mix the two kinds of single bacteria evenly according to the volume ratio of 1:2 to form a multi-functional composite bacterial agent, that is, a composite bacterial agent that has the functions of dissolving phosphorus and degrading DEHP.

[0043] (4) The composite bacterial agents, Pseudomonas prosekii YLYP6 and Rhodococcuspyridinivorans XB with equal numbers of bacteria were inoculated into NBRIP medium respectively, cultured at 30°C and 130rpm for 7 days, and each treatment was set up with 3 replicates.

[0044] (5) Determination of soluble phosphate: After culturing for 7 d...

Embodiment 3

[0046] Phosphorus-dissolving ability analysis of compound bacterial agent:

[0047] (1) Inoculate Pseudomonas prosekii YLYP6 and Rhodococcus pyridinivorans XB in LB liquid medium respectively, and activate at 30°C for 18 hours;

[0048] (2) Pick two activated single bacteria and inoculate them in LB liquid medium respectively, and culture them until the number of bacteria is 10 10 cfu / mL;

[0049] (3) Mix the two kinds of single bacteria evenly according to the volume ratio of 3:3 to form a multi-functional composite bacterial agent, that is, a composite bacterial agent that has the functions of dissolving phosphorus and degrading DEHP.

[0050] (4) Inoculate the compound bacterial agent, Pseudomonas prosekii YLYP6 and Rhodococcuspyridinivorans XB with the same number of bacteria into NBRIP medium respectively, and culture them in a sterile shaker at 30°C and 130 rpm for 7 days, and set up 3 replicates for each treatment.

[0051] (5) Determination of soluble phosphate: Afte...

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Abstract

The invention discloses a complex inoculant with phosphate solubilizing and DEHP (di(2-ethylhexyl)phthalate) degrading functions and an application of the complex inoculant, and belongs to the technical field of microorganisms. The complex inoculant comprises Pseudomonas prosekii YLYP6 and Rhodococcus pyridinivorans XB. The complex inoculant has better cooperativity, has the phosphate solubilizingand DEHP degrading functions, can improve phosphate solubilizing capacity and promote DEHP degradation, and plays an important role in improving phosphorus deficiency and soil contaminated by PAEs (phthalates); the complex inoculant is quick to reproduce and high in adaptability, adopts a simple and feasible culture method, has the application potential of improving nutrition status of soil and removing PAEs organic pollutants from soil and has great significance in culture and improvement of ecological fertility of soil, repair of contaminated soil, reduction of PAEs in crops, guarantee of crop quality safety and realization of simultaneous production and repair of farmland soil.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a composite bacterial agent capable of dissolving phosphorus and degrading DEHP and its application. Background technique [0002] Due to the single performance of a single microbial agent, weak adaptability, unstable effect, the complexity of the soil, water and other environments where the microbial agent is applied, and the increasingly urgent demand for multifunctional agents, it is urgent to use the agent Combining, cultivating and producing a multifunctional composite bacterial agent with stable function, strong adaptability and low price. Composite bacterial preparation is based on the theory of microecology and utilizes the combined effect of microbial flora to prepare live bacterial preparations from two or more beneficial and non-antagonistic microbial strains. It has a full range of types, reasonable compatibility, and functional Strong, high econom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14C12R1/38C12R1/01C09K101/00
CPCC09K17/14C09K2101/00C12N1/20
Inventor 莫测辉冯乃宪李彦文蔡全英喻乐意李慧赵海明
Owner JINAN UNIVERSITY
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