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Biological organic fertilizer with straw thoroughly decomposing effect and preparation method thereof

A technology of bio-organic fertilizer and decomposed straw, which is applied in the preparation of organic fertilizer, treatment of bio-organic parts, and organic fertilizer, etc., can solve the problems of single function of decomposer, long cycle, and influence on mechanical operation.

Inactive Publication Date: 2018-08-28
成都合成生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If relying solely on natural decomposing, the cycle is too long, which will affect the mechanical operation
[0003] The existing decomposing agent has a single effect and only has a decomposing effect on straw. Farmers need to add fertilizer in the later stage, which increases the frequency and intensity of operations.

Method used

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  • Biological organic fertilizer with straw thoroughly decomposing effect and preparation method thereof

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preparation example Construction

[0034] The present invention also provides a method for preparing bio-organic fertilizer with the effect of decomposing stalks, comprising the following steps:

[0035] Step 1, preparation of composite microbial fermentation inoculum:

[0036] Step 1.1, seed activation: use beef extract peptone medium for Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus lateralis, and Bacillus pumilus respectively, pick a ring from the seed test tube, and streak it on the eggplant bottle medium, 32 Cultivate at -35°C for 24-48 hours to obtain seeds cultured in shake flasks; use PDA medium for Pichia pastoris, pick a ring from the seed test tube, streak it on the eggplant bottle medium, and cultivate at 30-32°C for 24-48 hours , to obtain the seeds cultivated in shake flasks;

[0037] Step 1.2, expanded culture: cultured eggplant bottle strains, add sterile water to each eggplant bottle to make a bacterial suspension, make the concentration reach 10 billion / ml, add sterilized bacteria a...

Embodiment 1

[0052] A method for preparing bio-organic fertilizer with decomposed stalk effect, comprising the following steps:

[0053] Step 1, preparation of composite microbial fermentation inoculum:

[0054] Step 1.1, seed activation: use beef extract peptone medium for Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus lateralis, and Bacillus pumilus respectively, pick a ring from the seed test tube, and streak it on the eggplant bottle medium, 34 Cultivate at ℃ for 36 hours to obtain shake-flask-cultured seeds; Pichia pastoris uses PDA medium to pick a ring from the seed test tube, streak it on the eggplant bottle medium, and cultivate at 30°C for 36 hours to obtain shake-flask-cultured seeds;

[0055] Step 1.2, expanded culture: cultured eggplant bottle strains, add sterile water to each eggplant bottle to make a bacterial suspension, make the concentration reach 10 billion / ml, add sterilized bacteria according to the inoculation amount of 5%. In a 1000ml Erlenmeyer flask medi...

Embodiment 2

[0070] A method for preparing bio-organic fertilizer with decomposed stalk effect, comprising the following steps:

[0071] Step 1, preparation of composite microbial fermentation inoculum:

[0072] Step 1.1, seed activation: use beef extract peptone medium for Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus lateralis, and Bacillus pumilus respectively, pick a ring from the seed test tube, and streak it on the eggplant bottle medium, 32 Cultivate at ℃ for 48 hours to obtain shake-flask-cultured seeds; Pichia pastoris uses PDA medium to pick a ring from the seed test tube, streak it on the eggplant bottle medium, and cultivate at 30°C for 48 hours to obtain shake-flask-cultured seeds;

[0073] Step 1.2, expanded culture: cultured eggplant bottle strains, add sterile water to each eggplant bottle to make a bacterial suspension, make the concentration reach 10 billion / ml, add sterilized bacteria according to the inoculation amount of 5%. In a 1000ml Erlenmeyer flask medi...

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Abstract

The invention discloses a biological organic fertilizer with a straw thoroughly decomposing effect and a preparation method thereof. The biological organic fertilizer is prepared from the following raw materials in parts by weight: 35 to 45 parts of distiller's grain, 25 to 35 parts of animal dung, 10 to 26 parts of straw powder and 0.01 to 0.05 part of compound microbial fermentation inoculant. The biological organic fertilizer disclosed by the invention can improve soil structure and increase soil organic matter; meanwhile, microorganisms inoculated by early fermenting an organic fertilizercan mass propagate and exist in the organic fertilizer, straw decomposition is quickened, organic matter and beneficial elements are generated, and nutrient substances are provided for crop growth. Ina thoroughly decomposing process, metabolites generated by mass propagation of beneficial bacteria can generate bacteriostatic action on harmful germs in soil.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a bio-organic fertilizer with the effect of decomposing straw and a preparation method thereof. Background technique [0002] With the development of society, the improvement of living standards and environmental protection awareness, and the reduction of the application of chemical fertilizers, organic food is more and more popular among people. For a long time, people have used a large amount of chemical fertilizers in order to simply pursue yield, resulting in a rapid decline in soil fertility and an extreme imbalance of effective elements. Soil compaction, large accumulation of harmful metals and other hazards, thus exacerbating water and soil pollution. The application of organic fertilizers can increase the organic matter of the soil, and greatly improve the structure of the soil, nutrients, energy, enzymes, water, aeration and microbial activity in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/00
CPCC05F3/00C05F17/00C05F5/008C05F11/00C05F11/08Y02W30/40
Inventor 伍林
Owner 成都合成生物科技有限公司
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