Method used for simultaneous determination of nine functional components in bitter orange through ultra-high performance liquid chromatography-tandem mass spectrometry

Abstract

The invention relates to a detection method, and especially relates to a method used for simultaneous determination of nine functional components in bitter orange through ultra-high performance liquidchromatography-tandem mass spectrometry. The method comprises following steps: samples are subjected to separation so as to obtain a fruit peal part and a fruit pulp part, and methanol-water full extraction is carried out; Accucore XL C18 column (150*4mm, 4.6<mu>m) is adopted for separation, a methanol-0.15% formic acid solution is taken as a mobile phase for gradient elution, Electrospray Ionization Source positive and negative ion switching multiple- reaction monitoring mode tandem mass spectrum detection is adopted, and external standard method is adopted for quantification. It is shown inresults that the linear relationships of the nine functional components in relatively wide mass concentration ranges are excellent, the correlation coefficients are all larger than 0.997; the recovery rates of three additive amounts range from 83.57 to 112.91%; the relative standard deviations rang from 1.58 to 8.33% (n=6); four batches of citrus changshan-huyou samples are measured; and simultaneous determination of the nine functional components in the samples is carried out. The method is rapid, sensitive, and accurate, and is suitable for qualitative and quantitative determination of thenine functional components in citrus changshan-huyou.

Description

technical field [0001] The invention relates to a detection method, in particular to an ultra-high performance liquid chromatography-tandem mass spectrometry method for simultaneously determining nine functional components in Fructus aurantii. Background technique [0002] Citrus aurantium (zhǐ qiào) is a herbal medicine included in the "Chinese Pharmacopoeia", and its medicinal source is the dried immature fruit of Citrusaurantium L. and its cultivars of the Rutaceae plant. Harvest in July when the peel is still green, cut in half from the middle, and dry in the sun or at low temperature. Fructus aurantii is bitter, pungent, sour and warm in nature, and has the effects of regulating qi, widening the middle, moving stagnation and reducing swelling. disease. Fructus aurantium is produced in many places in my country, and products from Hunan and Jiangxi are the best. Among them, the Fructus aurantium in Xingan County, Jiangxi Province is also known as Shangzhou Fructus Fruc...

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Problems solved by technology

However, the simultaneous determination of multiple functional components in different parts of Citrus aurantium by ultra-high performance liquid chromatography-tandem mass spectrometry has not been reported in the literature.

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