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Method for reducing influence of -80 DEG C freeze storage on tumor killing activity of CIK cells

A cryopreservation and cell technology, applied in the field of cellular immunity, can solve the problems of decreased lethality of tumor cells and affecting the short-term preservation of CIK cells, and achieve the effect of reducing adverse effects

Inactive Publication Date: 2018-09-14
沈兵
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Problems solved by technology

Studies have shown that cryopreservation at -80°C for 3 months can basically maintain the killing activity of CIK cells on tumor cells, but from the 4th month onwards, the killing activity of CIK cells on tumor cells after recovery is significantly reduced (references: different frozen Effect of survival time on the immunophenotype and killing activity of CIK cells, Modern Oncology, Volume 24, Issue 10, May 2016)
[0003] The above-mentioned defects affect the effect of -80℃ cryopreservation on short-term preservation of CIK cells for 6 months

Method used

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  • Method for reducing influence of -80 DEG C freeze storage on tumor killing activity of CIK cells
  • Method for reducing influence of -80 DEG C freeze storage on tumor killing activity of CIK cells
  • Method for reducing influence of -80 DEG C freeze storage on tumor killing activity of CIK cells

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Embodiment Construction

[0030] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0031] 1. Experimental materials

[0032] Vitex diterpenoids B, C, D, E, begostin and 12-epi goshendin were purchased or self-made, and the purity was above 95%. The chemical structure and CAS registration number information are shown in the table below.

[0033]

[0034] RPMI 1640 medium was purchased from Gibco. PrimeScript RT Master Mix (reverse transcription kit) and SYBRPremix Ex Taq (fluorescence quantitative PCR kit) were purchased from Dalian Bao Biological Company.

[0035] 2. Experimental method

[0036] 1. CIK cell isolation, in vitro expansion, cryopreservation and recovery

[0037] Separation of CIK cells: collect peripheral anticoagulated whole blood from healthy volunteers, add an equal volume slowly along the tube wall into a 50ml centrifuge tube wit...

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Abstract

The invention discloses a method for reducing the influence of -80 DEG C freeze storage on the tumor killing activity of CIK cells. The effects that vitex diterpenes C, D and E or viscid germander herb can improve the tumor cell killing activity of the CIK cells after the -80 DEG C freeze storage of the CIK cells after 6 months through inhibiting the expression level of hsa-miR-543 in the CIK cells, and through the pretreatment by the vitex diterpenes C, D and E or viscid germander herb before the freeze storage, the tumor cell killing power of the CIK cells after the -80 DEG C for 6 months can be maintained at the level before the freeze storage are discovered. Therefore the method provided by the invention has the advantage that the adverse influence of the -80 DEG C freeze storage on the tumor killing activity of the CIK cells can be effectively reduced.

Description

technical field [0001] The invention belongs to the field of cellular immunity and relates to a method for reducing the tumor-killing activity of CIK cells cryopreserved at -80°C. Background technique [0002] Cryopreservation at -80°C is commonly used for short-term preservation of CIK cells within 6 months. Studies have shown that cryopreservation at -80°C for 3 months can basically maintain the killing activity of CIK cells on tumor cells, but from the 4th month onwards, the killing activity of CIK cells on tumor cells after recovery is significantly reduced (references: different frozen Effects of survival time on the immunophenotype and killing activity of CIK cells, Modern Oncology Medicine, Volume 24, Issue 10, May 2016). [0003] The above defects affect the short-term storage effect of CIK cells cryopreserved at -80°C for 6 months. Contents of the invention [0004] The invention aims to overcome the deficiencies of the prior art and provide a method for reducin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 沈兵
Owner 沈兵
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